Analysing the expression of eight clock genes in five tissues from fasting and fed sows
Cardoso, Tainã Figueiredo (Centre de Recerca en Agrigenòmica)
Quintanilla, Raquel (Institut de Recerca i Tecnologia Agroalimentàries)
Castelló Farré, Anna (Centre de Recerca en Agrigenòmica)
Mármol-Sánchez, Emilio (Centre de Recerca en Agrigenòmica)
Ballester Devis, Maria (Institut de Recerca i Tecnologia Agroalimentàries)
Jordana i Vidal, Jordi (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Amills i Eras, Marcel (Centre de Recerca en Agrigenòmica)

Fecha:	2018
Resumen:	In a previous study, we observed that circadian clock genes are differentially expressed in the skeletal muscle of fasting and fed sows. The goal of the current work was to investigate if these genes are also differentially expressed in tissues containing the central (hypothalamus) and peripheral (duodenum, dorsal fat, muscle, and liver) clocks. As animal material, we used 12 sows that fasted 12 h before slaughtering (T0) and 12 sows that were fed ad libitum 7 h prior slaughtering (T2). Tissue samples were collected immediately after slaughter and total RNA was subsequently extracted. The expression of the ARNTL, BHLHE40, CRY2, NPAS2, NR1D1, PER1, PER2, and SIK1 genes was measured by quantitative reverse transcription PCR. The numbers of clock genes showing differential expression before and after feeding varied depending on the tissue i. e. , four in dorsal fat and duodenum, six in skeletal muscle, and seven in the liver. In contrast, none of the eight analysed genes displayed a significant differential expression in hypothalamus, the tissue where the central clock resides. This result supports that the differential expression of clock genes in the four tissues mentioned above is probably induced by nutrition and not by the central clock entrained by light. Moreover, we have observed that the NPAS2 and ARNTL genes display positive log2(FC) values in the five tissues under analysis, whilst the CRY2, PER1 (except dorsal fat) and PER2 (except hypothalamus) genes generally show negative log2(FC) values. Such result might be explained by the existence of a negative feedback loop between the ARNTL/NPAS2 and CRY/PER genes. Collectively, these results support that nutrition plays an important role in modulating the timing of porcine peripheral circadian clocks. Such regulation could be essential for coordinating the subsequent metabolic response to nutrient supply.
Ayudas:	Ministerio de Economía y Competitividad AGL2013-48742-C2-1-R Ministerio de Economía y Competitividad AGL2013-48742-C2-2-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-1528 Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Educación y Ciencia FPU15-01733
Nota:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Clock genes ; Food ingestion ; Nutrition ; RT-qPCR ; Pig
Publicado en:	Frontiers in genetics, Vol. 9 (October 2018) , art. 475, ISSN 1664-8021

DOI: 10.3389/fgene.2018.00475
PMID: 30405688

8 p, 2.2 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > CRAG (Centro de Investigación en Agrigenómica)
Artículos > Artículos de investigación
Artículos > Artículos publicados