Web of Science: 3 cites, Scopus: 3 cites, Google Scholar: cites,
Analysing the expression of eight clock genes in five tissues from fasting and fed sows
Cardoso, Tainã Figueiredo (Centre de Recerca en Agrigenòmica)
Quintanilla, Raquel (Institut de Recerca i Tecnologia Agroalimentàries)
Castelló Farré, Anna (Centre de Recerca en Agrigenòmica)
Mármol-Sánchez, Emilio (Centre de Recerca en Agrigenòmica)
Ballester Devis, Maria (Institut de Recerca i Tecnologia Agroalimentàries)
Jordana i Vidal, Jordi (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Amills i Eras, Marcel (Centre de Recerca en Agrigenòmica)

Data: 2018
Resum: In a previous study, we observed that circadian clock genes are differentially expressed in the skeletal muscle of fasting and fed sows. The goal of the current work was to investigate if these genes are also differentially expressed in tissues containing the central (hypothalamus) and peripheral (duodenum, dorsal fat, muscle, and liver) clocks. As animal material, we used 12 sows that fasted 12 h before slaughtering (T0) and 12 sows that were fed ad libitum 7 h prior slaughtering (T2). Tissue samples were collected immediately after slaughter and total RNA was subsequently extracted. The expression of the ARNTL, BHLHE40, CRY2, NPAS2, NR1D1, PER1, PER2, and SIK1 genes was measured by quantitative reverse transcription PCR. The numbers of clock genes showing differential expression before and after feeding varied depending on the tissue i. e. , four in dorsal fat and duodenum, six in skeletal muscle, and seven in the liver. In contrast, none of the eight analysed genes displayed a significant differential expression in hypothalamus, the tissue where the central clock resides. This result supports that the differential expression of clock genes in the four tissues mentioned above is probably induced by nutrition and not by the central clock entrained by light. Moreover, we have observed that the NPAS2 and ARNTL genes display positive log2(FC) values in the five tissues under analysis, whilst the CRY2, PER1 (except dorsal fat) and PER2 (except hypothalamus) genes generally show negative log2(FC) values. Such result might be explained by the existence of a negative feedback loop between the ARNTL/NPAS2 and CRY/PER genes. Collectively, these results support that nutrition plays an important role in modulating the timing of porcine peripheral circadian clocks. Such regulation could be essential for coordinating the subsequent metabolic response to nutrient supply.
Ajuts: MINECO/AGL2013-48742-C2-1-R
Nota: Altres ajuts: CERCA Programme/Generalitat de Catalunya
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: Article ; recerca ; Versió publicada
Matèria: Clock genes ; Food ingestion ; Nutrition ; RT-qPCR ; Pig
Publicat a: Frontiers in genetics, Vol. 9 (October 2018) , art. 475, ISSN 1664-8021

DOI: 10.3389/fgene.2018.00475
PMID: 30405688

8 p, 2.2 MB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències > CRAG (Centre de Recerca en Agrigenòmica)
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 Registre creat el 2019-11-22, darrera modificació el 2021-08-01

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