Web of Science: 1 cites, Scopus: 1 cites, Google Scholar: cites,
The membrane proximal domain of TRPV1 and TRPV2 channels mediates protein-protein interactions and lipid binding in vitro
Doñate-Macian, Pablo (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Álvarez Marimon, Elena (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Sepulcre, Francesc (Universitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia)
Vázquez Ibar, José Luis (Institut des Sciences du Vivant Frédéric-JOLIOT. CEA-Saclay)
Perálvarez Marín, Alex (Universitat de Barcelona. Institut de Neurociències)

Data: 2019
Resum: Constitutive or regulated membrane protein trafficking is a key cell biology process. Transient receptor potential channels are somatosensory proteins in charge of detecting several physical and chemical stimuli, thus requiring fine vesicular trafficking. The membrane proximal or pre-S1 domain (MPD) is a highly conserved domain in transient receptor potential channels from the vanilloid (TRPV) subfamily. MPD shows traits corresponding to protein-protein and lipid-protein interactions, and protein regulatory regions. We have expressed MPD of TRPV1 and TRPV2 as green fluorescente protein (GFP)-fusion proteins to perform an in vitro biochemical and biophysical characterization. Pull-down experiments indicate that MPD recognizes and binds Soluble N-ethylmaleimide-sensitive factor Attachment Protein Receptors (SNARE). Synchrotron radiation scattering experiments show that this domain does not self-oligomerize. MPD interacts with phosphatidic acid (PA), a metabolite of the phospholipase D (PLD) pathway, in a specific manner as shown by lipid strips and Trp fluorescence quenching experiments. We show for the first time, to the best of our knowledge, the binding to PA of an N-terminus domain in TRPV channels. The presence of a PA binding domain in TRPV channels argues for putative PLD regulation. Findings in this study open new perspectives to understand the regulated and constitutive trafficking of TRPV channels exerted by protein-protein and lipid-protein interactions.
Ajuts: MICINN/SAF2010-21385
MICINN/BFU2017-87843-R
AGAUR/FI-2013FIB00251
European Commission 237120
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: Article ; recerca ; Versió publicada
Matèria: Transient Receptor Potential (TRP) channels ; Exocytosis ; Biophysics ; Protein-protein interactions ; Lipid-protein interactions
Publicat a: International journal of molecular sciences, Vol. 20, Issue 3 (February 2019) , art. 682, ISSN 1422-0067

DOI: 10.3390/ijms20030682
PMID: 30764505


11 p, 2.2 MB

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Articles > Articles publicats

 Registre creat el 2020-01-09, darrera modificació el 2021-08-01



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