Sterilization procedure for temperature-sensitive hydrogels loaded with silver nanoparticles for clinical applications
Rafael, Diana (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Andrade, Fernanda (Universidade do Porto. Instituto Nacional de Engenharia Biomédica)
Martinez-Trucharte, Francesc (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Basas Satorras, Jana (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Seras-Franzoso, Joaquin (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Palau Gauthier, Marta (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Gomis, Xavier (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Pérez-Burgos, Marc (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Blanco, Alberto (Hospital Universitari Vall d'Hebron)
López-Fernández, Alba (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Vélez, Roberto (Hospital Universitari Vall d'Hebron)
Abasolo, Ibane (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Aguirre Cañadell, M. (Màrius) (Màrius) (Hospital Universitari Vall d'Hebron)
Gavaldà, Joan (Hospital Universitari Vall d'Hebron)
Schwartz, Simon (Hospital Universitari Vall d'Hebron)
Universitat Autònoma de Barcelona

Data:	2019
Resum:	Hydrogels (HG) have recognized benefits as drug delivery platforms for biomedical applications. Their high sensitivity to sterilization processes is however one of the greatest challenges regarding their clinical translation. Concerning infection diseases, prevention of post-operatory related infections is crucial to ensure appropriate patient recovery and good clinical outcomes. Silver nanoparticles (AgNPs) have shown good antimicrobial properties but sustained release at the right place is required. Thus, we produced and characterized thermo-sensitive HG based on Pluronic® F127 loaded with AgNPs (HG-AgNPs) and their integrity and functionality after sterilization by dry-heat and autoclave methods were carefully assessed. The quality attributes of HG-AgNPs were seriously affected by dry-heat methods but not by autoclaving methods, which allowed to ensure the required sterility. Also, direct sterilization of the final HG-AgNPs product proved more effective than of the raw material, allowing simpler production procedures in non-sterile conditions. The mechanical properties were assessed in post mortem rat models and the HG-AgNPs were tested for its antimicrobial properties in vitro using extremely drug-resistant (XDR) clinical strains. The produced HG-AgNPs prove to be versatile, easy produced and cost-effective products, with activity against XDR strains and an adequate gelation time and spreadability features and optimal for in situ biomedical applications.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Hydrogels ; Poloxamer ; Silver nanopaticles ; Sterilization ; Anti-bacterial agents ; XDR strains ; Post-operative infecions ; Chirurgic applications
Publicat a:	Nanomaterials, Vol. 9 Núm. 3 (march 2019) , p. 380, ISSN 2079-4991

DOI: 10.3390/nano9030380
PMID: 30845683

14 p, 3.9 MB

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