A coordinated response at the transcriptome and interactome level is required to ensure uropathogenic escherichia coli survival during bacteremia
Sánchez de Groot, Natalia (Centre de Regulació Genòmica)
Torrent Burgas, Marc (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Data:	2019
Resum:	Localized infections or disruption of the skin barrier can enable the entry of bacteria into the bloodstream, possibly leading to acute inflammation and sepsis. There is currently no holistic view on how bacteria can survive and spread in the bloodstream. In this context, we combined transposon mutagenesis, gene-expression profiling and a protein interaction network analysis to examine how uropathogenic Escherichia coli can proliferate in blood. Our results indicate that, upon migration from the urea to serum, E. coli reacts to the osmolarity difference, triggering a transcriptomic response in order to express survival genes. The proteins codified by these genes are precisely organized at the interactome level and specifically target short linear motifs located in disordered regions of host proteins. Such a coordinated response helps to explain how bacteria can adapt to and survive environmental changes within the host. Overall, our results provide a general framework for the study of bacteremia and reveal new targets for potential study as novel antimicrobials.
Ajuts:	Ministerio de Economía y Competitividad RYC-2012-09999 Ministerio de Economía y Competitividad SAF2014-56568-R Ministerio de Economía y Competitividad SAF2015-72518-EXP Ministerio de Economía y Competitividad SAF2017-82158-R
Nota:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Escherichia coli ; Urinary tract infection ; Bacteremia ; Antimicrobials
Publicat a:	Microorganisms, Vol. 7, Issue 9 (September 2019) , art. 292, ISSN 2076-2607

Adreça alternativa: https://www.mdpi.com/2076-2607/7/9/292
DOI: 10.3390/microorganisms7090292
PMID: 31450662

12 p, 2.4 MB

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