Atmo-ecometabolomics : a novel atmospheric particle chemical characterization methodology for ecological research
Rivas-Ubach, Albert (Pacific Northwest National Laboratory (Richland, Estats Units))
Liu, Gina (Pacific Northwest National Laboratory (Richland, Estats Units))
Steiner, Allison L. (University of Michigan. Department of Climate and Space Sciences and Engineering)
Sardans i Galobart, Jordi (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Tfaily, Malak M. (Pacific Northwest National Laboratory (Richland, Estats Units))
Kulkarni, Gourihar (Pacific Northwest National Laboratorycific Northwest National Laboratory (Richland, Estats Units))
Kim, Young-Mo (Pacific Northwest National Laboratory (Richland, Estats Units))
Bourrianne, Eric (Université de Toulouse III Paul Sabatier. Faculté des Sicences et d'Ingénierie)
Paša-Tolić, Ljiljana (Pacific Northwest National Laboratory (Richland, Estats Units))
Peñuelas, Josep (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Guenther, Alex (University of California. Department of Earth System Science)

Data:	2019
Resum:	Aerosol particles play important roles in processes controlling the composition of the atmosphere and function of ecosystems. A better understanding of the composition of aerosol particles is beginning to be recognized as critical for ecological research to further comprehend the link between aerosols and ecosystems. While chemical characterization of aerosols has been practiced in the atmospheric science community, detailed methodology tailored to the needs of ecological research does not exist yet. In this study, we describe an efficient methodology (atmo-ecometabolomics), in step-by-step details, from the sampling to the data analyses, to characterize the chemical composition of aerosol particles, namely atmo-metabolome. This method employs mass spectrometry platforms such as liquid and gas chromatography mass spectrometries (MS) and Fourier transform ion cyclotron resonance MS (FT-ICR-MS). For methodology evaluation, we analyzed aerosol particles collected during two different seasons (spring and summer) in a low-biological-activity ecosystem. Additionally, to further validate our methodology, we analyzed aerosol particles collected in a more biologically active ecosystem during the pollination peaks of three different representative tree species. Our statistical results showed that our sampling and extraction methods are suitable for characterizing the atmo-ecometabolomes in these two distinct ecosystems with any of the analytical platforms. Datasets obtained from each mass spectrometry instrument showed overall significant differences of the atmo-ecometabolomes between spring and summer as well as between the three pollination peak periods. Furthermore, we have identified several metabolites that can be attributed to pollen and other plant-related aerosol particles. We additionally provide a basic guide of the potential use ecometabolomic techniques on different mass spectrometry platforms to accurately analyze the atmo-ecometabolomes for ecological studies. Our method represents an advanced novel approach for future studies in the impact of aerosol particle chemical compositions on ecosystem structure and function and biogeochemistry.
Ajuts:	European Commission 610028 Ministerio de Economía y Competitividad CGL2013-48074-P Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-274
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Aerosol particles ; Metabolomics ; Ecosystems ; Biomarkers ; Mass spectrometry ; FT-ICR
Publicat a:	Environmental monitoring and assessment, Vol. 191, issue 2 (Feb. 2019) , art. 78, ISSN 0167-6369

DOI: 10.1007/s10661-019-7205-x

Postprint 48 p, 2.2 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències > CREAF (Centre de Recerca Ecològica i d'Aplicacions Forestals) > Imbalance-P
Articles > Articles de recerca
Articles > Articles publicats