The Evening Complex Establishes Repressive Chromatin Domains Via H2A.Z Deposition
Tong, Meixuezi (University of Cambridge. Sainsbury Laboratory)
Lee, Kyounghee (Sungkyunkwan University. Department of Biological Sciences)
Ezer, Daphne (University of Cambridge. Sainsbury Laboratory)
Cortijo, Sandra (University of Cambridge. Sainsbury Laboratory)
Jung, Jaehoon (Sungkyunkwan University. Department of Biological Sciences)
Charoensawan, Varodom (University of Cambridge. Sainsbury Laboratory)
Box, Mathew S. (University of Cambridge. Sainsbury Laboratory)
Jaeger, Katja E. (University of Cambridge. Sainsbury Laboratory)
Takahashi, Nozomu (Centre de Recerca en Agrigenòmica)
Mas, Paloma (Centre de Recerca en Agrigenòmica)
Wigge, Philip Anthony (Leibniz-Institut für Gemüse- und Zierpflanzenbau)
Seo, Pil Joon (Seoul National University. Plant Genomics and Breeding Institute)

Data:	2020
Resum:	The Evening Complex (EC) is a core component of the Arabidopsis (Arabidopsis thaliana) circadian clock, which represses target gene expression at the end of the day and integrates temperature information to coordinate environmental and endogenous signals. Here we show that the EC induces repressive chromatin structure to regulate the evening transcriptome. The EC component ELF3 directly interacts with a protein from the SWI2/SNF2-RELATED (SWR1) complex to control deposition of H2A. Z-nucleosomes at the EC target genes. SWR1 components display circadian oscillation in gene expression with a peak at dusk. In turn, SWR1 is required for the circadian clockwork, as defects in SWR1 activity alter morning-expressed genes. The EC-SWR1 complex binds to the loci of the core clock genes PSEUDO-RESPONSE REGULATOR7 (PRR7) and PRR9 and catalyzes deposition of nucleosomes containing the histone variant H2A. Z coincident with the repression of these genes at dusk. This provides a mechanism by which the circadian clock temporally establishes repressive chromatin domains to shape oscillatory gene expression around dusk.
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Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Plant physiology, Vol. 182, Issue 1 (January 2020) , p. 612-625, ISSN 1532-2548

DOI: 10.1104/pp.19.00881
PMID: 31712305

14 p, 2.3 MB

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