Silencing against the conserved NAC domain of the potato StNAC103 reveals new NAC candidates to repress the suberin associated waxes in phellem
Soler, Marçal (Universitat de Girona. Laboratori del Suro)
Verdaguer, Roger (Universitat de Girona. Laboratori del Suro)
Fernández-Piñán, Sandra (Universitat de Girona. Laboratori del Suro)
Company-Arumí, Dolors (Universitat de Girona. Laboratori del Suro)
Boher, Pau (Universitat de Girona. Laboratori del Suro)
Góngora-Castillo, Elsa (Michigan State University. Department of Plant Biology)
Valls, Marc (Centre de Recerca en Agrigenòmica)
Anticó, Enriqueta (Universitat de Girona. Departament de Química)
Molinas, Marisa (Universitat de Girona. Laboratori del Suro)
Serra, Olga (Universitat de Girona. Laboratori del Suro)
Figueras, Mercè (Universitat de Girona. Laboratori del Suro)

Data:	2020
Resum:	Both suberin and its associated waxes contribute to the formation of apoplastic barriers that protect plants from the environment. Some transcription factors have emerged as regulators of the suberization process. The potato StNAC103 gene was reported as a repressor of suberin polyester and suberin-associated waxes deposition because its RNAi-mediated downregulation (StNAC103-RNAi) over-accumulated suberin and associated waxes in the tuber phellem concomitantly with the induction of representative biosynthetic genes. Here, to explore if other genes of the large NAC gene family participate to this repressive function, we extended the silencing to other NAC members by targeting the conserved NAC domain of StNAC103 (StNAC103-RNAi-c). Transcript profile of the StNAC103-RNAi-c phellem indicated that StNAC101 gene was an additional potential target. In comparison with StNAC103-RNAi, the silencing with StNAC103-RNAi-c construct resulted in a similar effect in suberin but yielded an increased load of associated waxes in tuber phellem, mainly alkanes and feruloyl esters. Globally, the chemical effects in both silenced lines are supported by the transcript accumulation profile of genes involved in the biosynthesis, transport and regulation of apoplastic lipids. In contrast, the genes of polyamine biosynthesis were downregulated. Altogether these results point out to StNAC101 as a candidate to repress the suberin-associated waxes.
Ajuts:	Ministerio de Educación y Ciencia AGL2006-07342 Ministerio de Economía y Competitividad AGL2012-36725 Ministerio de Economía y Competitividad AGL2015-67495-C2-1-R Ministerio de Ciencia e Innovación AGL2009-13745
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Suberin ; Suberin-associated waxes ; Periderm ; Phellem ; NAC transcription factors ; Solanum tuberosum
Publicat a:	Plant science, Vol. 291 (February 2020) , art. 110360, ISSN 1873-2259

DOI: 10.1016/j.plantsci.2019.110360

Postprint 32 p, 3.7 MB

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