A Species-Wide Inventory of NLR Genes and Alleles in Arabidopsis thaliana
Van de Weyer, Anna-Lena (Max Planck Institute for Developmental Biology. Department of Molecular Biology)
Monteiro, Freddy (Centre de Recerca en Agrigenòmica)
Furzer, Oliver J. (University of East Anglia. The Sainsbury Laboratory)
Nishimura, Marc T. (Colorado State University. Department of Biochemistry and Molecular Biology)
Cevik, Volkan (University of Bath. Milner Centre for Evolution & Department of Biology and Biochemistry)
Witek, Kamil (University of East Anglia. The Sainsbury Laboratory)
Jones, Jonathan D. G. (University of East Anglia. The Sainsbury Laboratory)
Dangl, Jeffery L. (Howard Hughes Medical Institute)
Weigel, Detlef (Max Planck Institute for Developmental Biology. Department of Molecular Biology)
Bemm, Felix (Max Planck Institute for Developmental Biology. Department of Molecular Biology)

Data:	2019
Resum:	Infectious disease is both a major force of selection in nature and a prime cause of yield loss in agriculture. In plants, disease resistance is often conferred by nucleotide-binding leucine-rich repeat (NLR) proteins, intracellular immune receptors that recognize pathogen proteins and their effects on the host. Consistent with extensive balancing and positive selection, NLRs are encoded by one of the most variable gene families in plants, but the true extent of intraspecific NLR diversity has been unclear. Here, we define a nearly complete species-wide pan-NLRome in Arabidopsis thaliana based on sequence enrichment and long-read sequencing. The pan-NLRome largely saturates with approximately 40 well-chosen wild strains, with half of the pan-NLRome being present in most accessions. We chart NLR architectural diversity, identify new architectures, and quantify selective forces that act on specific NLRs and NLR domains. Our study provides a blueprint for defining pan-NLRomes. In plants, NLR proteins are important intracellular receptors with roles in innate immunity and disease resistance. This work provides a panoramic view of this diverse and complicated gene family in the model species A. thaliana and provides a foundation for the identification and functional study of disease-resistance genes in agronomically important species with complex genomes.
Ajuts:	European Commission 340602
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Cell, Vol. 178, Issue 5 (August 2019) , p. 1260-1272, ISSN 1097-4172

DOI: 10.1016/j.cell.2019.07.038
PMID: 31442410

28 p, 5.4 MB

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