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Página principal > Artículos > Artículos publicados > Nanoscale structure of amyloid-β plaques in Alzheimer's disease |
Fecha: | 2019 |
Resumen: | Soluble amyloid-β (Aβ) is considered to be a critical component in the pathogenesis of Alzheimer's disease (AD). Evidence suggests that these non-fibrillar Aβ assemblies are implicated in synaptic dysfunction, neurodegeneration and cell death. However, characterization of these species comes mainly from studies in cellular or animal models, and there is little data in intact human samples due to the lack of adequate optical microscopic resolution to study these small structures. Here, to achieve super-resolution in all three dimensions, we applied Array Tomography (AT) and Stimulated Emission Depletion microscopy (STED), to characterize in postmortem human brain tissue non-fibrillar Aβ structures in amyloid plaques of cases with autosomal dominant and sporadic AD. Ultrathin sections scanned with super-resolution STED microscopy allowed the detection of small Aβ structures of the order of 100 nm. We reconstructed a whole human amyloid plaque and established that plaques are formed by a dense core of higher order Aβ species (~0. 022 µm ) and a peripheral halo of smaller Aβ structures (~0. 003 µm ). This work highlights the potential of AT-STED for human neuropathological studies. |
Ayudas: | Instituto de Salud Carlos III PI14-1561 Instituto de Salud Carlos III PI17-1896 Ministerio de Economía y Competitividad SEV-2015-0522 |
Nota: | Altres ajuts: PERIS/SLT002-16-00408-01 |
Derechos: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió publicada |
Materia: | Age of Onset ; Humans ; Middle Aged ; Nanoparticles ; Plaque ; Aged ; Alzheimer Disease ; Amyloid beta-Peptides ; Genes |
Publicado en: | Scientific reports, Vol. 9 Núm. 1 (january 2019) , p. 5181, ISSN 2045-2322 |
10 p, 10.3 MB |