Novel polyomaviruses in mammals from multiple orders and reassessment of polyomavirus evolution and taxonomy
Ehlers, Bernhard (Robert Koch Institute. Division 12 Measles, Mumps, Rubella and Viruses Affecting Immunocompromised Patients)
Anoh, Augustin E. (Université Alassane Ouattara. Centre de Recherche pour le Développement)
Salem, Nicole Ben (Robert Koch Institute. Division 12 Measles, Mumps, Rubella and Viruses Affecting Immunocompromised Patients)
Broll, Sebastian (Robert Koch Institute. Division 12 Measles, Mumps, Rubella and Viruses Affecting Immunocompromised Patients)
Couacy-Hymann, Emmanuel (Laboratoire National d'Appui Au Développement Agricole. Laboratoire Central de Pathologie Animale)
Fischer, Daniela (Robert Koch Institute. Division 12 Measles, Mumps, Rubella and Viruses Affecting Immunocompromised Patients)
Gedvilaite, Alma (Institute of Biotechnology. Vilnius University)
Ingenhütt, Nanine (Robert Koch Institute. Division 12 Measles, Mumps, Rubella and Viruses Affecting Immunocompromised Patients)
Liebmann, Sonja (Robert Koch Institute. Division 12 Measles, Mumps, Rubella and Viruses Affecting Immunocompromised Patients)
Martín, Maite (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Mossoun, Arsene (Laboratoire National d'Appui Au Développement Agricole. Laboratoire Central de Pathologie Animale)
Mugisha, Lawrence (Makerere University. College of Veterinary Medicine. Animal Resources and Biosecurity (COVAB))
Muyembe-Tamfum, Jena-Jacques (Institut National de Recherche Bio-Médicale)
Pauly, Maude (Robert Koch Institute. Epidemiology of Highly Pathogenic Microorganisms)
De Val, Bernat Pérez (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Preugschas, Hannah (Robert Koch Institute. Division 12 Measles, Mumps, Rubella and Viruses Affecting Immunocompromised Patients)
Richter, Dania (Technische Universität Braunschweig)
Schubert, Grit (Robert Koch Institute. Epidemiology of Highly Pathogenic Microorganisms)
Szentiks, Claudia A. (Leibniz Institute for Zoo and Wildlife Research (IZW))
Teichmann, Tamara (Robert Koch Institute. Division 12 Measles, Mumps, Rubella and Viruses Affecting Immunocompromised Patients)
Walter, Cornelia (Robert Koch Institute. Division 12 Measles, Mumps, Rubella and Viruses Affecting Immunocompromised Patients)
Ulrich, Rainer G. (Partner Site Hamburg-Lübeck-Borstel-Insel Riems. German Center for Infection Research (DZIF))
Wiersma, Lidewij (Robert Koch Institute. Epidemiology of Highly Pathogenic Microorganisms)
Leendertz, Fabian H. (Robert Koch Institute. Epidemiology of Highly Pathogenic Microorganisms)
Calvignac-Spencer, Sébastien (Robert Koch Institute. Viral Evolution)

Data:	2019
Resum:	As the phylogenetic organization of mammalian polyomaviruses is complex and currently incompletely resolved, we aimed at a deeper insight into their evolution by identifying polyomaviruses in host orders and families that have either rarely or not been studied. Sixteen unknown and two known polyomaviruses were identified in animals that belong to 5 orders, 16 genera, and 16 species. From 11 novel polyomaviruses, full genomes could be determined. Splice sites were predicted for large and small T antigen (LTAg, STAg) coding sequences (CDS) and examined experimentally in transfected cell culture. In addition, splice sites of seven published polyomaviruses were analyzed. Based on these data, LTAg and STAg annotations were corrected for 10/86 and 74/86 published polyomaviruses, respectively. For 25 polyomaviruses, a spliced middle T CDS was observed or predicted. Splice sites that likely indicate expression of additional, alternative T antigens, were experimentally detected for six polyomaviruses. In contrast to all other mammalian polyomaviruses, three closely related cetartiodactyl polyomaviruses display two introns within their LTAg CDS. In addition, the VP2 of Glis glis (edible dormouse) polyomavirus 1 was observed to be encoded by a spliced transcript, a unique experimental finding within the Polyomaviridae family. Co-phylogenetic analyses based on LTAg CDS revealed a measurable signal of codivergence when considering all mammalian polyomaviruses, most likely driven by relatively recent codivergence events. Lineage duplication was the only other process whose influence on polyomavirus evolution was unambiguous. Finally, our analyses suggest that an update of the taxonomy of the family is required, including the creation of novel genera of mammalian and non-mammalian polyomaviruses.
Nota:	Altres ajuts: DFG/LE1813/4-1
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Mamífers ; Malalties ; Polyomavirus ; Genome ; Evolution ; T antigen ; VP2 ; Splicing ; Taxonomy
Publicat a:	Viruses, Vol. 11 Núm. 10 (october 2019) , p. 930, ISSN 1999-4915

DOI: 10.3390/v11100930
PMID: 31658738

27 p, 2.2 MB

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