The fitness cost and benefit of phase-separated protein deposits
Sánchez de Groot, Natalia (Centre de Regulació Genòmica)
Torrent Burgas, Marc (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Ravarani, Charles N. J. (Medical Research Council Laboratory of Molecular Biology)
Trusina, Ala (Niels Bohr Institute)
Ventura, Salvador (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Babu, Madam M (Medical Research Council Laboratory of Molecular Biology)

Fecha:	2019
Resumen:	Phase separation of soluble proteins into insoluble deposits is associated with numerous diseases. However, protein deposits can also function as membrane-less compartments for many cellular processes. What are the fitness costs and benefits of forming such deposits in different conditions? Using a model protein that phase-separates into deposits, we distinguish and quantify the fitness contribution due to the loss or gain of protein function and deposit formation in yeast. The environmental condition and the cellular demand for the protein function emerge as key determinants of fitness. Protein deposit formation can influence cell-to-cell variation in free protein abundance between individuals of a cell population (i. e. , gene expression noise). This results in variable manifestation of protein function and a continuous range of phenotypes in a cell population, favoring survival of some individuals in certain environments. Thus, protein deposit formation by phase separation might be a mechanism to sense protein concentration in cells and to generate phenotypic variability. The selectable phenotypic variability, previously described for prions, could be a general property of proteins that can form phase-separated assemblies and may influence cell fitness.
Ayudas:	European Commission 330352 European Commission 299105 Ministerio de Economía y Competitividad SAF2017-82158-R Ministerio de Economía y Competitividad SAF2015-72518-EXP Ministerio de Economía y Competitividad RYC-2012-09999
Nota:	Altres ajuts: Beatriu de Pinos fellowships
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Cell fitness ; Natural selection ; Phase separation ; Phenotypic diversity ; Protein deposit
Publicado en:	Molecular systems biology, Vol. 15, issue 4 (April 2019) , art. e8075, ISSN 1744-4292

DOI: 10.15252/msb.20178075
PMID: 30962358

20 p, 3.5 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Instituto de Biotecnología y de Biomedicina (IBB)
Artículos > Artículos de investigación
Artículos > Artículos publicados