Non-invasive grading of astrocytic tumours from the relative contents of myo-inositol and glycine measured by in vivo MRS
Candiota Silveira, Ana Paula (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Majos, Carles (Hospital Universitari de Bellvitge. Institut de Diagnòstic per la Imatge)
Julià Sapé, Ma. Margarita (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Cabañas Egaña, Miquel (Universitat Autònoma de Barcelona. Servei de Ressonància Magnètica Nuclear)
Acebes Martín, Juan José (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Moreno Torres, Angel (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Griffiths, J. R. (Cancer Research UK. Cambridge Institute)
Arús i Caraltó, Carles (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Date:	2011
Abstract:	MRI and MRS are established methodologies for evaluating intracranial lesions. One MR spectral feature suggested for in vivo grading of astrocytic tumours is the apparent myo-Inositol (mI) intensity (ca 3. 55ppm) at short echo times, although glycine (gly) may also contribute in vivo to this resonance. The purpose of this study was to quantitatively evaluate the mI + gly contribution to the recorded spectral pattern in vivo and correlate it with in vitro data obtained from perchloric acid extraction of tumour biopsies. Patient spectra (n = 95) at 1. 5T at short (20-31 ms) and long (135-136 ms) echo times were obtained from the INTERPRET MRS database (http://gabrmn. uab. es/interpretvalidateddb/). Phantom spectra were acquired with a comparable protocol. Spectra were automatically processed and the ratios of the (mI + gly) to Cr peak heights ((mI + gly)/Cr) calculated. Perchloric acid extracts of brain tumour biopsies were analysed by high-resolution NMR at 9. 4T. The ratio (mI + gly)/Cr decreased significantly with astrocytic grade in vivo between low-grade astrocytoma (A2) and glioblastoma multiforme (GBM). In vitro results displayed a somewhat different tendency, with anaplastic astrocytomas having significantly higher (mI + gly)/Cr than A2 and GBM. The discrepancy between in vivo and in vitro data suggests that the NMR visibility of glycine in glial brain tumours is restricted in vivo.
Grants:	Ministerio de Ciencia e Innovación SAF008-03323 Ministerio de Educación y Ciencia SAF2005-03650 Ministerio de Ciencia y Tecnología SAF2002-00440 Agència de Gestió d'Ajuts Universitaris i de Recerca 2001/SGR-194 Agència de Gestió d'Ajuts Universitaris i de Recerca 2005/XT2002-48
Note:	Altres ajuts: INTERPRET (EU-IST1999-10310). This work was also partially funded by the Centro de Investigación Biomédica en Red - Bioingeniería, Biomateriales y Nanomedicina, which is an initiative of the Instituto de Salud Carlos III (Spain) co-funded by EU FEDER funds.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Astrocytoma - Magnetic resonance (MR) ; Spectroscopy
Published in:	Journal Belge de Radiologie - Belgisch Tijdschrift voor Radiologie, Vol. 94, issue 6 (2011) , p. 319-329, ISSN 1780-2393

DOI: 10.5334/jbr-btr.698

11 p, 315.5 KB

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