An enriched European eel transcriptome sheds light upon host-pathogen interactions with Vibrio vulnificus
Callol, Agnes (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Reyes-López, Felipe E. (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Roig, Francisco J. (Universitat de València. Departament de Microbiologia i Ecologia)
Goetz, Giles (Northwest Fisheries Science Center)
Goetz, Frederick W. (Northwest Fisheries Science Center)
Amaro, Carmen (Universitat de València. Departament de Microbiologia i Ecologia)
Mackenzie, Simon (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")

Date:	2015
Abstract:	Infectious diseases are one of the principal bottlenecks for the European eel recovery. The aim of this study was to develop a new molecular tool to be used in host-pathogen interaction experiments in the eel. To this end, we first stimulated adult eels with different pathogen- associated molecular patterns (PAMPs), extracted RNA from the immune-related tissues and sequenced the transcriptome. We obtained more than 2x10<sup>6</sup> reads that were assembled and annotated into 45,067 new descriptions with a notable representation of novel transcripts related with pathogen recognition, signal transduction and the immune response. Then, we designed a DNA-microarray that was used to analyze the early immune response against Vibrio vulnificus, a septicemic pathogen that uses the gills as the portal of entry into the blood, as well as the role of the main toxin of this species (RtxA1<inf>3</inf>) on this early interaction. The gill transcriptomic profiles obtained after bath infecting eels with the wild type strain or with a mutant deficient in rtxA1<inf>3</inf> were analyzed and compared. Results demonstrate that eels react rapidly and locally against the pathogen and that this immuneresponse is rtxA1<inf>3</inf>-dependent as transcripts related with cell destruction were highly up-regulated only in the gills from eels infected with the wild-type strain. Furthermore, significant differences in the immune response against the wild type and the mutant strain also suggest that host survival after V. vulnificus infection could depend on an efficient local phagocytic activity. Finally, we also found evidence of the presence of an interbranchial lymphoid tissue in European eel gills although further experiments will be necessary to identify such tissue.
Grants:	Ministerio de Ciencia e Innovación AGL2011-29639 Ministerio de Ciencia e Innovación CSD2009-00006 Ministerio de Ciencia e Innovación CSD2007-00002 Ministerio de Ciencia e Innovación BES-2009-024776
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons en que s'entrega l'obra al domini públic. Podeu copiar, modificar, distribuir l'obra i fer-ne comunicació pública, fins i tot amb finalitat comercial, sense demanar cap mena de permís.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Animals ; Eels ; Fish diseases ; Host-pathogen interactions ; Signal transduction ; Transcriptome ; Vibrio infections ; Vibrio vulnificus
Published in:	PloS one, Vol. 10, issue 7 (2015) , art. e0133328, ISSN 1932-6203

DOI: 10.1371/journal.pone.0133328
PMID: 26207370

20 p, 940.6 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles