Dynamic Interactions Between the Genome and an Endogenous Retrovirus : Tirant in Drosophila simulans Wild-Type Strains
Fablet, Marie (Université de Lyon. Laboratoire de Biométrie et Biologie Evolutive)
Jacquet, Angelo (Université de Lyon. Laboratoire de Biométrie et Biologie Evolutive)
Rebollo, Rita (Université de Lyon. INSA-Lyon, INRA)
Haudry, Annabelle (Université de Lyon. Laboratoire de Biométrie et Biologie Evolutive)
Rey, Carine (Université de Lyon. Laboratoire de Biologie et Modélisation de la Cellule)
Salces-Ortiz, Judit (Université de Lyon. Laboratoire de Biométrie et Biologie Evolutive)
Bajad, Prajakta (Medical University of Vienna. Center for Anatomy and Cell Biology, Division of Cell- and Developmental Biology)
Burlet, Nelly (Université de Lyon. Laboratoire de Biométrie et Biologie Evolutive)
Jantsch, Michael F. (Medical University of Vienna. Center for Anatomy and Cell Biology, Division of Cell- and Developmental Biology)
García Guerreiro, María Pilar (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Vieira, Cristina (Université de Lyon. Laboratoire de Biométrie et Biologie Evolutive)

Data:	2019
Resum:	All genomes contain repeated sequences that are known as transposable elements (TEs). Among these are endogenous retroviruses (ERVs), which are sequences similar to retroviruses and are transmitted across generations from parent to progeny. These sequences are controlled in genomes through epigenetic mechanisms. At the center of the epigenetic control of TEs are small interfering RNAs of the piRNA class, which trigger heterochromatinization of TE sequences. The tirant ERV of Drosophila simulans displays intra-specific variability in copy numbers, insertion sites, and transcription levels, providing us with a well-suited model to study the dynamic relationship between a TE family and the host genome through epigenetic mechanisms. We show that tirant transcript amounts and piRNA amounts are positively correlated in ovaries in normal conditions, unlike what was previously described following divergent crosses. In addition, we describe tirant insertion polymorphism in the genomes of three D. simulans wild-type strains, which reveals a limited number of insertions that may be associated with gene transcript level changes through heterochromatin spreading and have phenotypic impacts. Taken together, our results participate in the understanding of the equilibrium between the host genome and its TEs.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Transposable element ; Retrotransposon ; Drosophila ; Epigenetic control ; Pirna ; Chromatin
Publicat a:	G3, Vol. 9, Num. 3 (March 2019) , p. 855-865, ISSN 2160-1836

DOI: 10.1534/g3.118.200789
PMID: 30658967

11 p, 1.7 MB

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