Combined effects of spray-drying conditions and postdrying storage time and temperature on Salmonella choleraesuis and Salmonella typhimurium survival when inoculated in liquid porcine plasma
Blázquez Salvador, Elena (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Rodríguez, C. (APC EUROPE)
Ródenas, J. (APC EUROPE)
Saborido, N. (APC EUROPE)
Solà-Ginés, Marc (APC EUROPE)
Pérez de Rozas, Ana (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Campbell, Joy (APC Inc)
Segalés Coma, Joaquim (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Pujols, Joan (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Polo, Javier (APC Inc)

Date:	2018
Abstract:	The objective of this study was to determine the effectiveness of the spray-drying process on the inactivation of Salmonella choleraesuis and Salmonella typhimurium spiked in liquid porcine plasma and to test the additive effect of immediate postdrying storage. Commercial spray-dried porcine plasma was sterilized by irradiation and then reconstituted (1:9) with sterile water. Aliquots of reconstituted plasma were inoculated with either S. choleraesuis or S. typhimurium, subjected to spray-drying at an inlet temperature of 200°C and an outlet temperature of either 71 or 80°C, and each spray-drying temperature combinations were subjected to either 0, 30 or 60 s of residence time () as a simulation of residence time typical of commercial dryers. Spray-dried samples were stored at either 4·0 ± 3·0°C or 23·0 ± 0·3°C for 15 days. Bacterial counts of each Salmonella spp. , were completed for all samples. For both Salmonella spp. , spray-drying at both outlet temperatures reduced bacterial counts about 3 logs at 0 s, while there was about a 5·5 log reduction at 60 s. Storage of all dried samples at either 4·0 ± 3·0°C or 23·0 ± 0·3°C for 15 days eliminate all detectable bacterial counts of both Salmonella spp. Safety of raw materials from animal origin like spray-dried porcine plasma (SDPP) may be a concern for the swine industry. Spray-drying process and postdrying storage are good inactivation steps to reduce the bacterial load of Salmonella choleraesuis and Salmonella typhimurium. For both Salmonella spp. , spray-drying at 71°C or 80°C outlet temperatures reduced bacterial counts about 3 log at residence time (RT) 0 s, while there was about a 5. 5 log reduction at RT 60 s. Storage of all dried samples at either 4. 0 ± 3. 0°C or 23. 0 ± 0. 3°C for 15 days was effective for eliminating detectable bacterial counts of both Salmonella spp. Significance and Impact of the Study: Safety of raw materials from animal origin like spray-dried porcine plasma (SDPP) may be a concern for the swine industry. Spray-drying process and postdrying storage are good inactivation steps to reduce the bacterial load of Salmonella choleraesuis and Salmonella typhimurium. For both Salmonella spp. , spray-drying at 71°C or 80°C outlet temperatures reduced bacterial counts about 3 log at residence time (RT) 0 s, while there was about a 5. 5 log reduction at RT 60 s. Storage of all dried samples at either 4. 0 ± 3. 0°C or 23. 0 ± 0. 3°C for 15 days was effective for eliminating detectable bacterial counts of both Salmonella spp.
Note:	Altres ajuts: GC/2014DI066
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Salmonel·la ; Blood derivative ; Salmonella choleraesuis ; Salmonella typhimurium ; Spray-drying ; Storage conditions
Published in:	Letters in Applied Microbiology, Vol. 67 (june 2018) , p. 205-211, ISSN 1472-765X

DOI: 10.1111/lam.13017
PMID: 29889981

7 p, 316.4 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles