Molecular profile of urine extracellular vesicles from normo-functional kidneys reveal minimal differences between living and deceased donors
Lozano Ramos, Sara Inés (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Bancu, Ioana (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Carreras-Planella, Laura (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Monguió-Tortajada, Marta (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Cañas, Laura (Universitat Autònoma de Barcelona. Departament de Medicina)
Juega-Mariño, Javier (Universitat Autònoma de Barcelona. Departament de Medicina)
Bonet Sol, Josep (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Armengol Barnils, Maria del Pilar (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Lauzurica, Ricardo (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Borràs i Serres, Francesc Enric (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)

Date:	2018
Abstract:	Kidney transplantation (KTx) is the best therapeutic approach for chronic kidney diseases leading to irreversible kidney failure. Considering the origin of the graft, several studies have reported differences between living (LD) and deceased donors (DD) in graft and patient survival. These differences seem to be related to multiple factors including, donor age and time of cold ischemia among others. Many of transplanted organs come from old-aged DDs, in which pre-transplant biopsy is recommended. However, kidney biopsy has several limitations, and there is a need to develop alternatives to assess the status of a kidney before transplantation. As the analysis of urinary extracellular vesicles (uEVs) rendered promising results as non-invasive biomarkers of kidney-related pathologies, this pilot study aimed to investigate whether profiling uEVs of LDs and DDs may be of help to assess the quality of the kidney before nephrectomy. uEVs from 5 living donors and 7 deceased donors were isolated by size-exclusion chromatography, and their protein and miRNA content were analysed by liquid chromatography followed by mass spectrometry and next generation sequencing, respectively. Then, hierarchical clustering and venn diagrams were done with Perseus software and InteractiVenn tool. Specific EVs data bases were also used for Gene Ontology analysis. Next generation sequencing revealed that uEVs from DDs contained less miRNAs than LDs, but most of the DD-expressed miRNAs were shared with LDs (96%). Only miR-326 (targeting the apoptotic-related Bcl2) was found significantly over-represented in LD. Focusing on the protein content, we detected a low intra-group correlation in both types of donors. Despite these differences, hierarchical clustering of either miRNA or protein data could not identify a differential profile between LDs and DDs. Of note, 90% of transplanted patients had a functional graft after a year from KTx. In this pilot study we found that, in normo-functional grafts, minor differences in uEVs profile could not discriminate between LDs and DDs.
Grants:	Instituto de Salud Carlos III PI13-00050 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014SGR804 Instituto de Salud Carlos III FEDER/RD16-0009
Note:	Altres ajuts: This work was supported by the project, integrated in the National R + D + I and funded by the ISCIII and the European Regional Development Fund (http://www.isciii.es). Also by the "Suport Grups de Recerca" programme of Secretaria d'Universitats i Recerca, Dept. Empresa i Coneixement, Generalitat de Catalunya , Group REMAR, http://agaur.gencat.cat), the Instituto de Salud Carlos III-Red de Investigación Renal (REDinREN) (Feder Funds, http://www.isciii.es, http://redinren.org), and Fundació Cellex. FEB is sponsored by the "Researchers Stabilization Program" from the Spanish "Sistema Nacional de Salud" (SNS- ISCIII, http://www.isciii.es) and "Direcció d'Estratègia i Coordinació" Catalan Health Department (CES07/015).
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Extracellular vesicles ; Exosomes ; Kidney transplantation ; Kidney donor ; Size-exclusion chromatography
Published in:	BMC Nephrology, Vol. 19 (july 2018) , ISSN 1471-2369

DOI: 10.1186/s12882-018-0985-3
PMID: 30064375

11 p, 909.4 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles