The photoperiodic response of hypocotyl elongation involves regulation of CDF1 and CDF5 activity
Martín, Guiomar (Centre de Recerca en Agrigenòmica)
Veciana Grau, Núria (Centre de Recerca en Agrigenòmica)
Boix, Marc (Centre de Recerca en Agrigenòmica)
Rovira, Arnau (Centre de Recerca en Agrigenòmica)
Henriques, Rossana (Centre de Recerca en Agrigenòmica)
Monte Collado, Elena (Centre de Recerca en Agrigenòmica)

Data:	2020
Resum:	Hypocotyl elongation relies on directional cell expansion, a process under light and circadian clock control. Under short photoperiods (SD), hypocotyl elongation in Arabidopsis thaliana follows a rhythmic pattern, a process in which circadian morning-to-midnight waves of the transcriptional repressors PSEUDO-RESPONSE REGULATORS (PRRs) jointly gate PHYTOCHROME-INTERACTING FACTOR (PIF) activity to dawn. Previously, we described CYCLING DOF FACTOR 5 (CDF5) as a target of this antagonistic PRR/PIF dynamic interplay. Under SD, PIFs induce CDF5 accumulation specifically at dawn, when it promotes the expression of positive cell elongation regulators such as YUCCA8 to induce growth. In contrast to SD, hypocotyl elongation under long days (LD) is largely reduced. Here, we examine whether CDF5 is an actor in this photoperiod specific regulation. We report that transcription of CDF5 is robustly induced in SD compared to LD, in accordance with PIFs accumulating to higher levels in SD, and in contrast to other members of the CDF family, whose expression is mainly clock regulated and have similar waveforms in SD and LD. Notably, when CDF5 was constitutively expressed under LD, CDF5 protein accumulated to levels comparable to SD but was inactive in promoting cell elongation. Similar results were observed for CDF1. Our findings indicate that both CDFs can promote cell elongation specifically in shorter photoperiods, however, their activity in LD is inhibited at the post-translational level. These data not only expand our understanding of the biological role of CDF transcription factors, but also identify a previously unrecognized regulatory layer in the photoperiodic response of hypocotyl elongation.
Ajuts:	European Commission 334052 Ministerio de Economía y Competitividad BIO2015-68460-P Ministerio de Economía y Competitividad BIO2015-70812-ERC Ministerio de Economía y Competitividad BIO2015-72161-EXP Ministerio de Economía y Competitividad BSEV-2015-0533 Ministerio de Ciencia e Innovación RYC-2011-09220 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-718
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Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Publicat a:	Physiologia plantarum, Vol. 169, issue 3 (July 2020) , p. 480-490, ISSN 1399-3054

DOI: 10.1111/ppl.13119

Postprint 22 p, 1.6 MB

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