Increased PARP Activity and DNA Damage in NSCLC Patients : influence of COPD
Tang, Jun (Institut Hospital del Mar d'Investigacions Mèdiques)
Curull Serrano, Víctor (Institut Hospital del Mar d'Investigacions Mèdiques)
Wang, Xuejie (Institut Hospital del Mar d'Investigacions Mèdiques)
Ampurdanés, Coral (Institut Hospital del Mar d'Investigacions Mèdiques)
Duran-Jordà, Xavier (Institut Hospital del Mar d'Investigacions Mèdiques)
Pijuan, Lara (Institut Hospital del Mar d'Investigacions Mèdiques)
Rodríguez-Fuster, Alberto (Institut Hospital del Mar d'Investigacions Mèdiques)
Aguiló Espases, Rafael (Institut Hospital del Mar d'Investigacions Mèdiques)
Yélamos, José (Institut Hospital del Mar d'Investigacions Mèdiques)
Barreiro, Esther (Institut Hospital del Mar d'Investigacions Mèdiques)
Universitat Autònoma de Barcelona

Data:	2020
Resum:	(1) Background: Lung cancer (LC) is a major leading cause of death worldwide. Poly (ADP-ribose) polymerase (PARP)-1 and PARP-2 are key players in cancer. We aimed to assess PARP-1 and PARP-2 expression and activity and DNA damage in tumors and non-tumor lungs from patients with/without chronic obstructive pulmonary disease (COPD). (2) Methods: Lung tumor and non-tumor specimens were obtained through video-assisted thoracoscopic surgery (VATS) in LC patients with/without underlying COPD (two groups of patients, n = 15/group). PARP-1 and PARP-2 expression (ELISA), PARP activity (PARP colorimetric assay kit) and DNA damage (immunohistochemistry) levels were identified in all samples. (3) Results: Both PARP-1 and PARP-2 expression levels were significantly lower in lung tumors (irrespective of COPD)compared to non-tumor specimens, while DNA damage and PARP activity levels significantly increased in lung tumors compared to non-tumor specimens only in LC-COPD patients. PARP-2 expression was positively correlated with smoking burden in LC-COPD patients. (4) Conclusions: In lung tumors of COPD patients, an overactivation of PARP enzyme was observed. A decline in PARP-1 and PARP-2 protein expression was seen in lung tumors irrespective of COPD. Other phenotypic features (airway obstruction) beyond cancer may account for the increase in PARP activity seen in the tumors of patients with underlying COPD.
Ajuts:	Instituto de Salud Carlos III FIS 18-00075
Nota:	Altres ajuts: This study was funded by FEDER, ISC-III & CIBERES, SEPAR 2018 & SEPAR 2020.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Lung cancer ; DNA damage ; PARP-1 and PARP-2 expression ; PARP activity ; COPD
Publicat a:	Cancers, Vol. 12 Núm. 11 (2020) , p. 3333, ISSN 2072-6694

DOI: 10.3390/cancers12113333
PMID: 33187221

13 p, 23.3 MB

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