Potential of MMP-9 based nanoparticles at optimizing the cow dry period : pulling apart the effects of MMP-9 and nanoparticles
Gifre-Renom, Laia (Institut de Recerca i Tecnologia Agroalimentàries)
Carratalá, José Vicente (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Parés Riera, Sílvia (Institut de Recerca i Tecnologia Agroalimentàries)
Sánchez-García, Laura (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Ferrer-Miralles, Neus (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Bach, Àlex (Institut de Recerca i Tecnologia Agroalimentàries)
Garcia-Fruitos, Elena (Institut de Recerca i Tecnologia Agroalimentàries)
Arís i Giralt, Anna (Institut de Recerca i Tecnologia Agroalimentàries)

Date:	2020
Abstract:	The cow dry period is a non-milking interval where the mammary gland involutes and regenerates to guarantee an optimal milk production in the subsequent lactation. Important bottlenecks such as the high risk of intramammary infections complicate the process. Antibiotics have been routinely used as a preventive treatment but the concerns about potential antibiotic resistance open a new scenario in which alternative strategies have to be developed. Matrix metalloproteinase-9 (MMP-9) is an enzyme able to degrade the extracellular matrix, triggering the involution and immune function of cow mammary gland. We have studied the infusion into the mammary gland of MMP-9 inclusion bodies as protein-based nanoparticles, demonstrating that 1. 2 mg of MMP-9 enhanced the involution and immune function of the cow mammary gland. However, the comparison of the effects triggered by the administration of an active and an inactive form of MMP-9 led to conclude that the response observed in the bovine mammary gland was mainly due to the protein format but not to the biological activity of the MMP-9 embedded in the inclusion body. This study provides relevant information on the future use of protein inclusion bodies in cow mammary gland and the role of MMP-9 at dry-off.
Grants:	Ministerio de Economía y Competitividad RTA2012-00028-C02-01 Ministerio de Economía y Competitividad RTA2012-00028-C02-02 Ministerio de Economía y Competitividad RTA2015-00064-C02-01 Ministerio de Economía y Competitividad RTA2015-00064-C02-02 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229
Note:	Altres ajuts: ICREA Academia award
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Animal biotechnology ; Disease prevention ; Nanobiotechnology
Published in:	Scientific reports, Vol. 10 (July 2020) , art. 11299, ISSN 2045-2322

DOI: 10.1038/s41598-020-67176-2
PMID: 32647244

8 p, 1.4 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles