Plant buffering against the high-light stress-induced accumulation of CsGA2ox8 transcripts via alternative splicing to finely tune gibberellin levels and maintain hypocotyl elongation
Liu, Bin (Centre de Recerca en Agrigenòmica)
Zhao, Shuo (Shanghai Jiao Tong University. School of Agriculture and Biology)
Li, Pengli (Shanghai Jiao Tong University. School of Agriculture and Biology)
Yin, Yilu (Shanghai Jiao Tong University. School of Agriculture and Biology)
Niu, Qingliang (Shanghai Jiao Tong University. School of Agriculture and Biology)
Yan, Jinqiang (Guangdong Academy of Agricultural Sciences)
Huang, Danfeng (Shanghai Jiao Tong University. School of Agriculture and Biology)

Data:	2021
Resum:	In plants, alternative splicing (AS) is markedly induced in response to environmental stresses, but it is unclear why plants generate multiple transcripts under stress conditions. In this study, RNA-seq was performed to identify AS events in cucumber seedlings grown under different light intensities. We identified a novel transcript of the gibberellin (GA)-deactivating enzyme Gibberellin 2-beta-dioxygenase 8 (CsGA2ox8). Compared with canonical CsGA2ox8. 1, the CsGA2ox8. 2 isoform presented intron retention between the second and third exons. Functional analysis proved that the transcript of CsGA2ox8. 1 but not CsGA2ox8. 2 played a role in the deactivation of bioactive GAs. Moreover, expression analysis demonstrated that both transcripts were upregulated by increased light intensity, but the expression level of CsGA2ox8. 1 increased slowly when the light intensity was >400 µmol·m −2 ·s −1 PPFD (photosynthetic photon flux density), while the CsGA2ox8. 2 transcript levels increased rapidly when the light intensity was >200 µmol·m −2 ·s −1 PPFD. Our findings provide evidence that plants might finely tune their GA levels by buffering against the normal transcripts of CsGA2ox8 through AS.
Nota:	Ajuts: this study was supported by The National Key Research and Development Program of China (2019YFD1000300), the International Postdoctoral Exchange Fellowship Program from the China Postdoctoral Council (20170053), the Technology System Construction of Modern Agricultural Industry of Shanghai (19Z113040008), and the Presidential Foundation of Guangdong Academy of Agricultural Sciences (BZ201901).
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Light responses ; RNA splicing
Publicat a:	Horticulture research, Vol. 8, art. 2 (Jan. 2021) , ISSN 2052-7276

DOI: 10.1038/s41438-020-00430-w
PMID: 33384414

10 p, 3.6 MB

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