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Pàgina inicial > Articles > Articles publicats > Improving biomaterials imaging for nanotechnology : |
Data: | 2018 |
Resum: | The preparation of biological samples for electron microscopy is material- and time-consuming because it is often based on long protocols that also may produce artifacts. Protein labeling for transmission electron microscopy (TEM) is such an example, taking several days. However, for protein-based nanotechnology, high resolution imaging techniques are unique and crucial tools for studying the spatial distribution of these molecules, either alone or as components of biomaterials. In this paper, we tested two new short methods of immunolocalization for TEM, and compared them with a standard protocol in qualitative and quantitative approaches by using four protein-based nanoparticles. We reported a significant increase of labeling per area of nanoparticle in both new methodologies (H = 19. 811; p < 0. 001) with all the model antigens tested: GFP (H = 22. 115; p < 0. 001), MMP-2 (H = 19. 579; p < 0. 001), MMP-9 (H = 7. 567; p < 0. 023), and IFN-γ (H = 62. 110; p < 0. 001). We also found that the most suitable protocol for labeling depends on the nanoparticle's tendency to aggregate. Moreover, the shorter methods reduce artifacts, time (by 30%), residues, and reagents hindering, losing, or altering antigens, and obtaining a significant increase of protein localization (of about 200%). Overall, this study makes a step forward in the development of optimized protocols for the nanoscale localization of peptides and proteins within new biomaterials. |
Ajuts: | Ministerio de Economía y Competitividad RTA2012-00028-C02-02 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-132 |
Nota: | Altres ajuts: AV thanks the granting of an ICREA ACADEMIA award. EGF thanks CERCA Programme (Generalitat de Catalunya), and European Social Fund for supporting our research. OCG received a PhD fellowship from MECD (FPU) and EGF a post-doctoral fellowship from INIA (DOC-INIA). The authors also acknowledge Micalis Institute INRA, France that kindly provides us the strain clpP-hrtA- NZ9000 (patent n°EP1141337B1/US6994997B1). |
Drets: | Tots els drets reservats. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió acceptada per publicar |
Publicat a: | Biotechnology Journal, Vol. 13, issue 4 (April 2018) , art. 1700388, ISSN 1860-7314 |
Postprint 21 p, 363.5 KB |