A genome-wide association study reveals cytokinin as a major component in the root defense responses against Ralstonia solanacearum
Alonso-Díaz, Alejandro (Centre de Recerca en Agrigenòmica)
Satbhai, Santosh B. (Austrian Academy of Sciences. Gregor Mendel Institute (Austria))
de Pedro Jové, Roger (Centre de Recerca en Agrigenòmica)
Berry, Hannah M. (Colorado State University. Cell and Molecular Biology (USA))
Göschl, Christian (Austrian Academy of Sciences. Gregor Mendel Institute (Austria))
Argueso, Cristiana T. (Colorado State University. Department of Bioagricultural Sciences and Pest Management (USA))
Novak, Ondrej (Laboratory of Growth Regulators (The Czech Republic))
Busch, Wolfgang (Salk Institute for Biological Studies (La Jolla, Estats Units d'Amèrica). Plant Molecular and Cellular Biology Laboratory)
Valls, Marc (Centre de Recerca en Agrigenòmica)
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)

Data:	2021
Resum:	Bacterial wilt caused by the soil-borne pathogen Ralstonia solancearum is economically devastating, with no effective methods to fight the disease. This pathogen invades plants through their roots and colonizes their xylem, clogging the vasculature and causing rapid wilting. Key to preventing colonization are the early defense responses triggered in the host's root upon infection, which remain mostly unknown. Here, we have taken advantage of a high-throughput in vitro infection system to screen natural variability associated with the root growth inhibition phenotype caused by R. solanacearum in Arabidopsis during the first hours of infection. To analyze the genetic determinants of this trait, we have performed a genome-wide association study, identifying allelic variation at several loci related to cytokinin metabolism, including genes responsible for biosynthesis and degradation of cytokinin. Further, our data clearly demonstrate that cytokinin signaling is induced early during the infection process and cytokinin contributes to immunity against R. solanacearum. This study highlights a new role for cytokinin in root immunity, paving the way for future research that will help in understanding the mechanisms underpinning root defenses.
Ajuts:	Agencia Estatal de Investigación PID2019-105416RB-I00 Ministerio de Economía y Competitividad RyC 2014-16158 Ministerio de Economía y Competitividad AGL2016-78002-R Ministerio de Economía y Competitividad SEV-2015-0533
Nota:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Bacterial wilt ; Cytokinin ; Defense ; GWAS ; Hormones ; Immune system ; Ralstonia solanacearum ; Root ; Salicylic acid
Publicat a:	Journal of experimental botany, Vol. 72, Issue 7 (March 2021) , p. 2727-2740, ISSN 1460-2431

DOI: 10.1093/jxb/eraa610
PMID: 33475698

14 p, 2.4 MB

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