Second- and third-generation biodiesel production with immobilised recombinant Rhizopus oryzae lipase : Influence of the support, substrate acidity and bioprocess scale-up
López Fernández, Josu (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Data:	2021
Resum:	Rhizopus oryzae lipase immobilised onto differently functionalised polymethacrylate (Purolite®) and magnetite superparamagnetic supports was assessed as a catalyst for biodiesel production with pomace oil. The presence of surface hydrocarbon chains increased the operational stability of the biocatalysts supported on Purolite® and superparamagnetic particles up to 9 and 2 times, respectively. By contrast, the presence of functional groups had no effect on the initial transesterification rate, which was twice higher with the lipase immobilised onto Purolite®. Also, functionalising Purolite® with epoxide and octadecyl groups led to the highest biodiesel and volumetric productivity. This biocatalyst with other substrates including makauba, jatropha, waste cooking oil, and microbial oil, led to similar initial reaction rates. However, simply raising substrate acidity from 0. 5 to 2% increased the operational stability of the biocatalysts 15 times. A synergistic effect between acyl-acceptor concentration and substrate acidity was observed. The transesterification reaction was successfully scaled up to 50 mL.
Ajuts:	Ministerio de Ciencia e Innovación PID2019-104666GB-100
Nota:	Altres ajuts: Beca del Gobierno Vasco para la formación de investigadores predoctorales PRE_2017_1_0110
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Biodiesel ; Biocatalysis ; Rhizopus oryzae lipase ; Immobilisation ; Substrate acidity
Publicat a:	Bioresource Technology, Vol. 334 (August 2021) , art. 125233, ISSN 1873-2976

DOI: 10.1016/j.biortech.2021.125233

9 p, 4.2 MB

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