Nitric oxide-dependent biodegradation of graphene oxide reduces inflammation in the gastrointestinal tract
Peng, Guotao (Karolinska Institutet (Estocolm, Suècia). Institute of Environmental Medicine)
Montenegro, Marcelo F. (Karolinska Institutet (Estocolm, Suècia). Department of Physiology and Pharmacology)
Ntola, Chifundo N. M. (University of Manchester. National Graphene Institute)
Vranic, Sandra (University of Manchester. National Graphene Institute)
Kostarelos, Kostas (Institut Català de Nanociència i Nanotecnologia)
Vogt, Carmen (Kungl. Tekniska högskolan (Estocolm (Suècia))
Toprak, Muhammet S. (Kungl. Tekniska högskolan (Estocolm (Suècia))
Duan, Tianbo (Uppsala University. Department of Engineering Sciences)
Leifer, Klaus (Uppsala University. Department of Engineering Sciences)
Bräutigam, Lars (Karolinska Institutet (Estocolm, Suècia))
Lundberg, Jon O. (Karolinska Institutet (Estocolm, Suècia). Department of Physiology and Pharmacology)
Fadeel, Bengt (Karolinska Institutet (Estocolm, Suècia). Institute of Environmental Medicine)

Data:	2020
Resum:	Understanding the biological fate of graphene-based materials such as graphene oxide (GO) is crucial to assess adverse effects following intentional or inadvertent exposure. Here we provide first evidence of biodegradation of GO in the gastrointestinal tract using zebrafish as a model. Raman mapping was deployed to assess biodegradation. The degradation was blocked upon knockdown of nos2a encoding the inducible nitric oxide synthase (iNOS) or by pharmacological inhibition of NOS using L-NAME, demonstrating that the process was nitric oxide (NO)-dependent. NOdependent degradation of GO was further confirmed in vitro by combining a superoxide-generating system, xanthine/xanthine oxidase (X/XO), with an NO donor (PAPA NONOate), or by simultaneously producing superoxide and NO by decomposition of SIN-1. Finally, by using the transgenic strain Tg(mpx:eGFP) to visualize the movement of neutrophils, we could show that inhibition of the degradation of GO resulted in increased neutrophil infiltration into the gastrointestinal tract, indicative of inflammation.
Ajuts:	European Commission 785219
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Adverse effect ; Gastrointestinal tract ; Generating system ; Inducible nitric oxide synthase (iNOS) ; Neutrophil infiltration ; Pharmacological inhibition ; Raman mapping ; Transgenic strains ; Animals ; Gastrointestinal Tract ; Graphite ; Inflammation ; Nitric Oxide ; Nitric Oxide Synthase ; Nitric Oxide Synthase Type II ; Zebrafish
Publicat a:	Nanoscale, Vol. 12, Issue 32 (August 2020) , p. 16730-16737, ISSN 2040-3372

DOI: 10.1039/d0nr03675g

8 p, 4.7 MB

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