![]() |
|||||||||||||||
![]() |
Cerca | Lliura | Ajuda | Servei de Biblioteques | Sobre el DDD | Català English Español |
Pàgina inicial > Articles > Articles publicats > Bacteria Detection at a Single-Cell Level through a Cyanotype-Based Photochemical Reaction |
Data: | 2021 |
Resum: | The detection of living organisms at very low concentrations is necessary for the early diagnosis of bacterial infections, but it is still challenging as there is a need for signal amplification. Cell culture, nucleic acid amplification, or nanostructure-based signal enhancement are the most common amplification methods, relying on long, tedious, complex, or expensive procedures. Here, we present a cyanotype-based photochemical amplification reaction enabling the detection of low bacterial concentrations up to a single-cell level. Photocatalysis is induced with visible light and requires bacterial metabolism of iron-based compounds to produce Prussian Blue. Bacterial activity is thus detected through the formation of an observable blue precipitate within 3 h of the reaction, which corresponds to the concentration of living organisms. The short time-to-result and simplicity of the reaction are expected to strongly impact the clinical diagnosis of infectious diseases. |
Ajuts: | Fundació la Marató de TV3 2018-201825-30-31 European Commission 675412 Agencia Estatal de Investigación PID2019-106171RB-I00 Ministerio de Economía y Competitividad SAF2015-67476-R Agencia Estatal de Investigación RTI2018-096278-B-C21 |
Nota: | Altres ajuts: acords transformatius de la UAB |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Bacteria detection ; Bacterial infections ; Early diagnosis ; Living organisms ; Low concentrations ; Nucleic acids amplification ; Photochemicals ; Signal amplifications ; Signal enhancement ; Single-cell level |
Publicat a: | Analytical chemistry, Vol. 94, Issue 2 (January 2021) , p. 787-792, ISSN 1520-6882 |
6 p, 4.8 MB |