Web of Science: 2 cites, Scopus: 2 cites, Google Scholar: cites,
Ex vivo exposure to different types of graphene-based nanomaterials consistently alters human blood secretome
Ballesteros Ribera, Sandra (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Domenech, Josefa (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Velázquez, Antonia (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Marcos Dauder, Ricardo (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Hernández Bonilla, Alba (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)

Data: 2021
Resum: The biomedical applications of graphene-based nanomaterials (GBN) have significantly grown in the last years. Many of these applications suppose their intravenous exposure and, in this way, GBN could encounter blood cells triggering an immunological response of unknown effects. Consequently, understanding the relationships between GBN and the immune system response should be a prerequisite for its adequate use in biomedicine. In the present study, we have conducted a little explored ex vivo exposure method in order to study the complexity of the secretome given by the interactions between GBN and blood cells. Blood samples from different healthy donors were exposed to three different types of GBN widely used in the biomedical field. In this sense, graphene oxide (GO), graphene nanoplatelets (GNPs), graphene nanoribbons (GNRs) and a panel of 105 proteins representatives of the blood secretome were evaluated. The results show broad changes in both the cytokines number and the expression levels, with important changes in inflammatory response markers. Furthermore, the indirect soft-agar assay was used as a tool to unravel the global functional impact of the found secretome changes. Our results indicate that the GBN-induced altered secretome can modify the natural anchorage-independent growth capacity of HeLa cells, used as a model. As a conclusion, this study describes an innovative approach to study the potential harmful effects of GBN, providing relevant data to be considered in the biomedical context when GBN are planned to be used in patients.
Ajuts: Ministerio de Educación, Cultura y Deporte BFU2016-76831-R
Nota: Altres ajuts: acords transformatius de la UAB
Nota: We wish to thank Mr. C. Valiente for his technical assistance. This work was partially supported by the Spanish Ministry of Education and Science [ BFU2016-76831-R ]. Sandra Ballesteros holds a fellowship from Universitat Autònoma de Barcelona [PIF-UAB].
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Llengua: Anglès
Document: Article ; recerca ; Versió publicada
Matèria: Graphene-based-nanomaterials ; Blood secretome ; Ex vivo exposure ; Cytokines ; Inflammation
Publicat a: Journal of hazardous materials, Vol. 414 (2021) , p. 125471, ISSN 1873-3336

DOI: 10.1016/j.jhazmat.2021.125471
PMID: 33647622

9 p, 4.7 MB

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