Rabbit hemorrhagic disease virus capsid, a versatile platform for foreign B-cell epitope display inducing protective humoral immune responses
Moreno, Noelia (Centro de Investigación en Sanidad Animal. CISA (Madrid, Espanya))
Mena, Ignacio (Centro de Investigación en Sanidad Animal. CISA (Madrid, Espanya))
Angulo-Chacón, Iván (Centro de Investigación en Sanidad Animal. CISA (Madrid, Espanya))
Gómez, Yolanda (Centro de Investigación en Sanidad Animal. CISA (Madrid, Espanya))
Crisci, Elisa (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Montoya, Maria (The Pirbright Institute (Regne Unit))
Castón, José R. (Centro Nacional de Biotecnología. Department of Structure of Macromolecules)
Blanco Lavilla, Esther (Centro de Investigación en Sanidad Animal. CISA (Madrid, Espanya))
Bárcena, Juan (Centro de Investigación en Sanidad Animal. CISA (Madrid, Espanya))

Date:	2016
Abstract:	Virus-like particles (VLPs), comprised of viral structural proteins devoid of genetic material, are tunable nanoparticles that can be chemically or genetically engineered, to be used as platforms for multimeric display of foreign antigens. Here, we report the engineering of chimeric VLPs, derived from rabbit hemorrhagic disease virus (RHDV) for presentation of foreign B-cell antigens to the immune system. The RHDV capsid comprises 180 copies of a single capsid subunit (VP60). To evaluate the ability of chimeric RHDV VLPs to elicit protective humoral responses against foreign antigens, we tested two B-cell epitopes: a novel neutralizing B-cell epitope, derived from feline calicivirus capsid protein, and a well characterized B-cell epitope from the extracellular domain of influenza A virus M2 protein (M2e). We generated sets of chimeric RHDV VLPs by insertion of the foreign B-cell epitopes at three different locations within VP60 protein (which involved different levels of surface accessibility) and in different copy numbers per site. The immunogenic potential of the chimeric VLPs was analyzed in the mouse model. The results presented here indicated that chimeric RHDV VLPs elicit potent protective humoral responses against displayed foreign B-cell epitopes, demonstrated by both, in vitro neutralization and in vivo protection against a lethal challenge.
Grants:	Ministerio de Economía y Competitividad AGL2013-48923-C2-1-R Ministerio de Economía y Competitividad BFU2014-55475R
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Scientific reports, Vol. 6 (08 2016) , ISSN 2045-2322

DOI: 10.1038/srep31844
PMID: 27549017

14 p, 2.2 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles