Biochemical and proteomic analyses of the physiological response induced by individual housing in gilts provide new potential stress markers
Marco-Ramell, Anna (Universitat de Barcelona. Departament de Nutrició, Ciències de l'Alimentació i Gastronomia)
Arroyo, Laura. (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Peña, Raquel (Universitat Autònoma de Barcelona. Servei de Bioquímica Clínica Veterinària)
Pato, Raquel (Universitat Autònoma de Barcelona. Servei de Bioquímica Clínica Veterinària)
Saco Rodriguez, Yolanda (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Fraile, Lorenzo (Agrotecnio Center)
Bendixen, Emøke (Aarhus University. Department of Molecular Biology and Genetics)
Bassols Teixidó, Anna Maria (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Data:	2016
Resum:	The objective assessment of animal stress and welfare requires proper laboratory biomarkers. In this work, we have analyzed the changes in serum composition in gilts after switching their housing, from pen to individual stalls, which is generally accepted to cause animal discomfort. Blood and saliva samples were collected a day before and up to four days after changing the housing system. Biochemical analyses showed adaptive changes in lipid and protein metabolism after the housing switch, whereas cortisol and muscular markers showed a large variability between animals. 2D-DIGE and iTRAQ proteomic approaches revealed variations in serum protein composition after changing housing and diet of gilts. Both techniques showed alterations in two main homeostatic mechanisms: the innate immune and redox systems. The acute phase proteins haptoglobin, apolipoprotein A-I and α1-antichymotrypsin 3, and the antioxidant enzyme peroxiredoxin 2 were found differentially expressed by 2D-DIGE. Other proteins related to the innate immune system, including lactotransferrin, protegrin 3 and galectin 1 were also identified by iTRAQ, as well as oxidative stress enzymes such as peroxiredoxin 2 and glutathione peroxidase 3. Proteomics also revealed the decrease of apolipoproteins, and the presence of intracellular proteins in serum, which may indicate physical injury to tissues. Housing of gilts in individual stalls and diet change increase lipid and protein catabolism, oxidative stress, activate the innate immune system and cause a certain degree of tissue damage. We propose that valuable assays for stress assessment in gilts may be based on a score composed by a combination of salivary cortisol, lipid metabolites, innate immunity and oxidative stress markers and intracellular proteins. The online version of this article (doi:10. 1186/s12917-016-0887-1) contains supplementary material, which is available to authorized users.
Ajuts:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2014SGR-4 Ministerio de Economía y Competitividad AGL2011-30598-C03-02
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Acute phase proteins ; Biomarker ; Individual confinement ; Innate immunity ; Lipids ; Oxidative stress ; Pig ; Proteomics ; Stress
Publicat a:	BMC veterinary research, Vol. 12 (november 2016) , ISSN 1746-6148

DOI: 10.1186/s12917-016-0887-1
PMID: 27887661

11 p, 599.0 KB

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