A Rapid Test for Soy Aeroallergens Exposure Assessment
Álvarez-Simón, Daniel (Universitat Autònoma de Barcelona. Departament de Medicina)
Cruz Carmona, María Jesús (Universitat Autònoma de Barcelona. Departament de Medicina)
Untoria, María-Dolores (Hospital Universitari Vall d'Hebron)
Muñoz Gall, Xavier (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Villalbí, Joan Ramón (Institut d'Investigació Biomèdica Sant Pau)
Morell Brotad, Ferran 1944- (Universitat Autònoma de Barcelona. Departament de Medicina)
Gómez-Ollés, Susana (Universitat Autònoma de Barcelona. Departament de Medicina)

Data:	2014
Resum:	Determining soy aeroallergens levels is extremely important in the assessment of health risks due to these airborne substances. Currently, soy aeroallergens exposure in the environment is monitored using enzyme immunoassays (EIA) which must be evaluated in a specialized laboratory by skilled personnel. To describe the development and performance of a rapid immunochromatography assay for the detection of soy aeroallergens in environmental samples. A test strip using gold labeled anti-soy hull low molecular weight extract (SHLMWE) antibody for the rapid detection of soy aeroallergens in environmental samples was developed. One hundred nineteen airborne samples were analysed in parallel by the strip assay and the anti-SHLMWE sandwich EIA. The assay results were visually analysed by three independent observers who ranked samples as: -, + or ++. Strips were also scanned and analysed by densitometry. The rapid test detected a range of concentrations from 6. 25 to 25 ng/mL. Agreement in strip assay interpretations between evaluators was substantial (Kappa = 0. 63; CI 0. 544-0. 715). Visual interpretation also gave a good concordance with EIA results, with sensitivity ranging from 77. 3 to 100 and specificity from 65 to 83. 5 depending on the observer. Furthermore, a strong correlation was observed between densitometry results of strip assay and EIA determinations. The strip assay developed is rapid, simple, and sensitive and does not require expensive equipment or specific skills. It has considerable potential in the environmental monitoring field for screening soy aeroallergens levels in port cities where allergen measurements are not currently performed. Due to its simplicity, the test will improve the management of soy allergic patients by controlling environmental allergen exposure without the need for apparatus or skilled personnel.
Ajuts:	Ministerio de Economía y Competitividad CP12/03101
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 9, Issue 2 (February 2014) , art. e88676, ISSN 1932-6203

DOI: 10.1371/journal.pone.0088676
PMID: 24533134

7 p, 799.9 KB

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