Regulatory Lymphocytes Are Key Factors in MHC-Independent Resistance to EAE
Marín, Nieves (Red Española de Esclerosis Múltiple)
Mecha, Miriam (Instituto Cajal (Consejo Superior de Investigaciones Científicas))
Espejo, Carmen (Universitat Autònoma de Barcelona)
Mestre, Leyre (Instituto Cajal (Consejo Superior de Investigaciones Científicas))
Eixarch, Herena (Universitat Autònoma de Barcelona)
Montalban, Xavier (Universitat Autònoma de Barcelona)
Alvarez-Cermeño, José C (Universidad de Alcalá)
Guaza, Carmen (Instituto Cajal (Consejo Superior de Investigaciones Científicas))
Villar, Luisa M. (Red Española de Esclerosis Múltiple)

Data:	2014
Resum:	Background and Objectives. Resistant and susceptible mouse strains to experimental autoimmune encephalomyelitis (EAE), an inducible demyelinating experimental disease serving as animal model for multiple sclerosis, have been described. We aimed to explore MHC-independent mechanisms inducing resistance to EAE. Methods. For EAE induction, female C57BL/6 (susceptible strain) and CD1 (resistant outbred strain showing heterogeneous MHC antigens) mice were immunized with the 35-55 peptide of myelin oligodendrocyte glycoprotein (MOG). We studied T cell proliferation, regulatory and effector cell subpopulations, intracellular and serum cytokine patterns, and titers of anti-MOG serum antibodies. Results. Upon immunization with MOG, T lymphocytes from susceptible mice but not that of resistant strain were capable of proliferating when stimulated with MOG. Accordingly, resistant mice experienced a rise in regulatory B cells (P = 0. 001) and, to a lower extent, in regulatory T cells (P = 0. 02) compared with C57BL/6 susceptible mice. As a consequence, MOG-immunized C57BL/6 mice showed higher percentages of CD4+ T cells producing both IFN-gamma (P = 0. 02) and IL-17 (P = 0. 009) and higher serum levels of IL-17 (P = 0. 04) than resistant mice. Conclusions. Expansion of regulatory B and T cells contributes to the induction of resistance to EAE by an MHC-independent mechanism.
Ajuts:	Ministerio de Ciencia e Innovación PS09/01652 Ministerio de Ciencia e Innovación PS09/01338 Ministerio de Ciencia e Innovación SAF2010/17501 Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-0793 Ministerio de Ciencia e Innovación CD09/00363 Ministerio de Ciencia e Innovación RD07/0060 Ministerio de Economía y Competitividad CPII13/00028 Ministerio de Sanidad y Consumo CP07/00146
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Journal of Immunology Research, Vol. 2014 (april 2014) , ISSN 2314-7156

DOI: 10.1155/2014/156380
PMID: 24868560

11 p, 2.5 MB

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