Web of Science: 2 cites, Scopus: 2 cites, Google Scholar: cites,
Rat Middle Cerebral Artery Occlusion Is Not a Suitable Model for the Study of Stroke-Induced Spontaneous Infections
Campos Martorell, Mireia (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Hernandez Guillamon, Maria Mar (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Rosell Novel, Anna (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Gomis Rodríguez, Javier (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Salat, David (Hospital Universitari Vall d'Hebron. Institut de Recerca)
García Bonilla, Lídia (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Montaner, Joan (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona

Data: 2014
Resum: Infections related to stroke-induced immunodepression are an important complication causing a high rate of death in patients. Several experimental studies in mouse stroke models have described this process but it has never been tested in other species such as rats. Our study focused on the appearance of secondary systemic and pulmonary infections in ischemic rats, comparing with sham and naive animals. For that purpose, male Wistar rats were subjected to embolic middle cerebral artery occlusion (eMCAO) or to transient MCAO (tMCAO) inserting a nylon filament. Forty-eight hours after ischemia, blood and lung samples were evaluated. In eMCAO set, ischemic rats showed a significant decrease in blood-peripheral lymphocytes (naive = 58. 8±18. 1%, ischemic = 22. 9±16. 4%) together with an increase in polymorphonuclears (PMNs) (naive = 29. 2±14. 7%, ischemic = 71. 7±19. 5%), while no change in monocytes was observed. The increase in PMNs counts was positively correlated with worse neurological outcome 48 hours after eMCAO (r = 0. 55, p = 0. 043). However, sham animals showed similar changes in peripheral leukocytes as those seen in ischemic rats (lymphocytes: 40. 1±19. 7%; PMNs: 51. 7±19. 2%). Analysis of bacteriological lung growth showed clear differences between naive (0±0 CFU/mL; log10) and both sham (3. 9±2. 5 CFU/mL; log10) and ischemic (4. 3±2. 9 CFU/mL; log10) groups. Additionally, naive animals presented non-pathological lung histology, while both sham and ischemic showed congestion, edema or hemorrhage. Concordant results were found in the second set of animals submitted to a tMCAO. Inflammatory and infection changes in Wistar rats subjected to MCAO models may be attributed not only to the brain ischemic injury but to the surgical aggression and/or anaesthetic stress. Consequently, we suggest that stroke-induced immunodepression in ischemic experimental models should be interpreted with caution in further experimental and translational studies, at least in rat stroke models that entail cervicotomy and cranial trepanation.
Ajuts: Ministerio de Economía y Competitividad FI 10/00508
Ministerio de Economía y Competitividad P09/00265
Ministerio de Ciencia e Innovación CP12/03259
Ministerio de Ciencia e Innovación RD12/0014/0005
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: Article ; recerca ; Versió publicada
Publicat a: PloS one, Vol. 9 (june 2014) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0099169
PMID: 24922549


9 p, 4.2 MB

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