Immunogenicity of 60 novel latency-related antigens of Mycobacterium tuberculosis
Serra-Vidal, Mᵃdel Mar (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Latorre, Irene (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Franken, Kees L. C. M. (Department of Immunohematology and Blood Transfusion/Department of Infectious Diseases, Leiden University Medical Center)
Díaz, Jéssica (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
De Souza Galvão, Maria Luiza (Hospital Universitari Vall d'Hebron)
Casas, Irma (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Maldonado, José (Serveis Clínics (Barcelona))
Milà, Celia (Hospital Universitari Vall d'Hebron)
Solsona, Jordi (Hospital Universitari Vall d'Hebron)
Jimenez-Fuentes, M. Ángeles (Hospital Universitari Vall d'Hebron)
Altet, Neus (Hospital Universitari Vall d'Hebron)
Lacoma, Alicia (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Ruiz-Manzano, Juan (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Ausina, Vicente (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Prat i Aymerich, Cristina (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Ottenhoff, Tom H. M. (Department of Immunohematology and Blood Transfusion/Department of Infectious Diseases, Leiden University Medical Center)
Domínguez, José (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)

Date:	2014
Abstract:	The aim of our work here was to evaluate the immunogenicity of 60 mycobacterial antigens, some of which have not been previously assessed, notably a novel series of in vivo -expressed Mycobacterium tuberculosis (IVE-TB) antigens. We enrolled 505 subjects and separated them in individuals with and without latent tuberculosis infection (LTBI) vs. patients with active tuberculosis (TB). Following an overnight and 7 days stimulation of whole blood with purified recombinant M. tuberculosis antigens, interferon-γ (IFN-γ) levels were determined by ELISA. Several antigens could statistically significantly differentiate the groups of individuals. We obtained promising antigens from all studied antigen groups [dormancy survival regulon (DosR regulon) encoded antigens; resuscitation-promoting factors (Rpf) antigens; IVE-TB antigens; reactivation associated antigens]. Rv1733, which is a probable conserved transmembrane protein encoded in DosR regulon, turned out to be very immunogenic and able to discriminate between the three defined TB status, thus considered a candidate biomarker. Rv2389 and Rv2435n, belonging to Rpf family and IVE-TB group of antigens, respectively, also stood out as LTBI biomarkers. Although more studies are needed to support our findings, the combined use of these antigens would be an interesting approach to TB immunodiagnosis candidates.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Tuberculosis ; Latent tuberculosis infection ; Immune response ; Antigenic stimulation ; Interferon-γ
Published in:	Frontiers in microbiology, Vol. 5 (october 2014) , ISSN 1664-302X

DOI: 10.3389/fmicb.2014.00517
PMID: 25339944

13 p, 1.6 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles