Reference Intervals of Thyroid Function Tests Assessed by Immunoassay and Mass Spectrometry in Healthy Pregnant Women Living in Catalonia
Hernández, José María (Institut Germans Trias i Pujol)
Soldevila i Madorell, Berta (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Velasco, Inés (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Moreno-Flores, Fernando (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Ferrer, Laura (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pérez-Montes de Oca, Alejandra (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Santillán, Cecilia (Hospital Doctor Arturo Oñativia)
Muñoz, Carla (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Ballesta Purroy, Sílvia (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Canal Girol, Cristina (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Puig Domingo, Manuel (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Granada, Maria Luisa (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Universitat Autònoma de Barcelona

Data:	2021
Resum:	Background: Recent guidelines recommend establishing a local reference interval (RI) for thyroid function. We aimed to establish trimester-specific RIs for thyrotropin (TSH) and free thyroxine (FT4) in a cohort of healthy pregnant women in Catalonia (Spain). Methods: A prospective observational study was conducted with 332 healthy pregnant women, from the first trimester (1T) to delivery. TSH was measured using an Architect ® immunoassay (Abbott) and FT4 by two immunoassays, Architect ® (Abbott) and Cobas ® (Roche), in the three trimesters. FT4 was also measured by liquid chromatography mass spectrometry (LC/MS/MS) in the 1T. Results: TSH (µUI/mL) increased throughout pregnancy (1T: 0. 03-3. 78; 2T: 0. 51-3. 53; 3T: 0. 50-4. 32; p < 0. 0001) and FT4 (pmol/L) progressively decreased (Architect ® 1T: 10. 42-15. 96; 2T: 8. 37-12. 74; 3T: 8. 24-12. 49; p < 0. 0001; and Cobas ® : 1T: 11. 46-19. 05; 2T: 9. 65-14. 67; 3T: 8. 88-14. 54; p < 0. 0067). The FT4 RI during 1T determined LC/MS/MS was 8. 75-18. 27. Despite the 1T FT4 results measured by LC/MS/MS and with the two immunoassays being significantly correlated, the results obtained by the three methods were found to be non-interchangeable. Conclusions: We established trimester-specific RIs for TSH and for FT4 with immunoassays in our population. We also validated the 1T FT4 using LC/MS/MS to confirm the results of FT4 lower than the 2. 5th percentile or higher than the 97. 5th percentile.
Ajuts:	Ministerio de Economía y Competitividad PI15/02192
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Immunoassays ; Pregnancy ; Reference intervals ; Tandem mass ; Thyroid function
Publicat a:	Journal of clinical medicine, Vol. 10 (may 2021) , ISSN 2077-0383

DOI: 10.3390/jcm10112444
PMID: 34072898

10 p, 1.1 MB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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