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Pàgina inicial > Articles > Articles publicats > The Poly-Histidine Tag H6 Mediates Structural and Functional Properties of Disintegrating, Protein-Releasing Inclusion Bodies |
Data: | 2022 |
Resum: | The coordination between histidine-rich peptides and divalent cations supports the formation of nano- and micro-scale protein biomaterials, including toxic and non-toxic functional amyloids, which can be adapted as drug delivery systems. Among them, inclusion bodies (IBs) formed in recombinant bacteria have shown promise as protein depots for time-sustained protein release. We have demonstrated here that the hexahistidine (H6) tag, fused to recombinant proteins, impacts both on the formation of bacterial IBs and on the conformation of the IB-forming protein, which shows a higher content of cross-beta intermolecular interactions in H6-tagged versions. Additionally, the addition of EDTA during the spontaneous disintegration of isolated IBs largely affects the protein leakage rate, again protein release being stimulated in His-tagged materials. This event depends on the number of His residues but irrespective of the location of the tag in the protein, as it occurs in either C-tagged or N-tagged proteins. The architectonic role of H6 in the formation of bacterial IBs, probably through coordination with divalent cations, offers an easy approach to manipulate protein leakage and to tailor the applicability of this material as a secretory amyloidal depot in different biomedical interfaces. In addition, the findings also offer a model to finely investigate, in a simple set-up, the mechanics of protein release from functional secretory amyloids. |
Ajuts: | Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229 Agencia Estatal de Investigación PID2019-105416RB-I00 Agencia Estatal de Investigación PID2019-107298RB-C22 Instituto de Salud Carlos III CP19/00028 Ministerio de Ciencia e Innovación FPU18/04615 Agència de Gestió d'Ajuts Universitaris i de Recerca 2019/FI_B00352 |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; Versió publicada |
Matèria: | Inclusion bodies ; Functional amyloids ; Protein secretion ; His-cation coordination ; Biomaterials ; Protein materials ; Slow protein release ; Drug delivery |
Publicat a: | Pharmaceutics, Vol. 14, Issue 3 (March 2022) , art. 602, ISSN 1999-4923 |
12 p, 2.0 MB |