When Phosphatases Go Mad : the Molecular Basis for Toxicity of Yeast Ppz1
Casamayor Gracia, Antonio (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Ariño Carmona, Joaquín (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")

Date:	2022
Abstract:	The fact that overexpression of the yeast Ser/Thr protein phosphatase Ppz1 induces a dramatic halt in cell proliferation was known long ago, but only work in the last few years has provided insight into the molecular basis for this toxicity. Overexpression of Ppz1 causes abundant changes in gene expression and modifies the phosphorylation state of more than 150 proteins, including key signaling protein kinases such as Hog1 or Snf1. Diverse cellular processes are altered: halt in translation, failure to properly adapt to low glucose supply, acidification of the cytosol, or depletion of intracellular potassium content are a few examples. Therefore, the toxicity derived from an excess of Ppz1 appears to be multifactorial, the characteristic cell growth blockage thus arising from the combination of various altered processes. Notably, overexpression of the Ppz1 regulatory subunit Hal3 fully counteracts the toxic effects of the phosphatase, and this process involves intracellular relocation of the phosphatase to internal membranes.
Grants:	Ministerio de Economía y Competitividad BFU2014-54591-C2-1-P Agencia Estatal de Investigación BFU2017-82574-P
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; Versió publicada
Subject:	Protein phosphatases ; Protein overexpression ; Transcriptomics ; Phosphoproteomics ; Intracellular signaling ; PH homeostasis ; Saccharomyces cerevisiae
Published in:	International journal of molecular sciences, Vol. 23, Issue 8 (April 2022) , art. 4304, ISSN 1422-0067

Adreça alternativa: https://www.mdpi.com/1422-0067/23/8/4304
DOI: 10.3390/ijms23084304
PMID: 35457140

15 p, 1.1 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Published articles