| Date: |
2020 |
| Description: |
42 pag. |
| Abstract: |
Progression towards active tuberculosis (TB) has been linked to the formation of Neutrophilic Extracellular Traps (NETs) in mammals. This study evaluated the role of this mechanism in the TB model of Drosophila Melanogaster (Drosophila) after Mycobacterium marinum (M. marinum) inoculation in third instar larvae. After bacterial injection, in vivo and in vitro tracking of inflammatory cell dynamics associated with the infection was performed. To monitor the dynamics, the binary UAS-GAL4 system was used to create specific gain-of-function phenotypes. Notably, the filopodial formation was seen in both in vitro and in vivo samples, indicating morphological similarities with NETs formation. In vitro, filopodial extensions were associated with M. marinum bacteria, which suggests functional similarities with NETs. However, no DNA stained with DAPI was detected outside of the nuclei neither in viable cells nor disrupted cells, demonstrating that Drosophila larvae may not produce Hemocyte Extracellular Traps (HETs) after bacterial infection. |
| Note: |
TFM |
| Note: |
One Health |
| Rights: |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan aquestes es distribueixin sota la mateixa llicència que regula l'obra original i es reconegui l'autoria de l'obra original.  |
| Language: |
Anglès |
| Studies: |
Zoonosi i Una Sola Salut (One Health) [4315915] |
| Study plan: |
Màster Universitari en Zoonosi i Una Sola Salut (One Health) [1460] |
| Document: |
Treball de fi de postgrau |
| Subject area: |
Veterinària |
| Subject: |
Tuberculosi |
guest ::
dir.
