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| Pàgina inicial > Articles > Articles publicats > Polylactide, Processed by a Foaming Method Using Compressed Freon R134a, for Tissue Engineering |
(Institut de Ciència de Materials de Barcelona) (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí") (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina) (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina) (Instituto de Investigación Sanitaria del Hospital Universitario La Paz) (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
| Data: | 2021 |
| Resum: | Fabricating polymeric scaffolds using cost-effective manufacturing processes is still challenging. Gas foaming techniques using supercritical carbon dioxide (scCO) have attracted attention for producing synthetic polymer matrices; however, the high-pressure requirements are often a technological barrier for its widespread use. Compressed 1,1,1,2-tetrafluoroethane, known as Freon R134a, offers advantages over CO in manufacturing processes in terms of lower pressure and temperature conditions and the use of low-cost equipment. Here, we report for the first time the use of Freon R134a for generating porous polymer matrices, specifically polylactide (PLA). PLA scaffolds processed with Freon R134a exhibited larger pore sizes, and total porosity, and appropriate mechanical properties compared with those achieved by scCO processing. PLGA scaffolds processed with Freon R134a were highly porous and showed a relatively fragile structure. Human mesenchymal stem cells (MSCs) attached to PLA scaffolds processed with Freon R134a, and their metabolic activity increased during culturing. In addition, MSCs displayed spread morphology on the PLA scaffolds processed with Freon R134a, with a well-organized actin cytoskeleton and a dense matrix of fibronectin fibrils. Functionalization of Freon R134a-processed PLA scaffolds with protein nanoparticles, used as bioactive factors, enhanced the scaffolds' cytocompatibility. These findings indicate that gas foaming using compressed Freon R134a could represent a cost-effective and environmentally friendly fabrication technology to produce polymeric scaffolds for tissue engineering approaches. |
| Ajuts: | Ministerio de Economía y Competitividad PI15/00752 Ministerio de Economía y Competitividad PI15/01118 Ministerio de Economía y Competitividad MAT2016-80826-R Agencia Estatal de Investigación RTI2018-095159-B-I00 Agencia Estatal de Investigación PID2019-105622RB-I00 Instituto de Salud Carlos III PI18/00643 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-918 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229 Fundació la Marató de TV3 201812 |
| Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.  |
| Llengua: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Matèria: | 3D scaffolds ; Biomaterial engineering ; Tissue engineering ; Mesenchymal stem cells ; Polymeric foams ; Surface functionalization ; Protein nanoparticles ; Cell growth ; Compressed fluids ; Freon R134a |
| Publicat a: | Polymers, Vol. 13, Num. 20 (October 2021) , art. 3453, ISSN 2073-4360 |
