Low n-6/n-3 Gestation and Lactation Diets Influence Early Performance, Muscle and Adipose Polyunsaturated Fatty Acid Content and Deposition, and Relative Abundance of Proteins in Suckling Piglets
Manaig, Yron Joseph (Centre de Recerca en Agrigenòmica)
Sandrini, Silvia (Università degli Studi di Milano. Department of Veterinary Medicine and Animal Sciences)
Panseri, Sara (Università degli Studi di Milano. Department of Veterinary Medicine and Animal Sciences)
Tedeschi, Gabriella (Università degli Studi di Milano. CRC Innovation for Well-Being and Environment)
Folch, Josep M. (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Sánchez Bonastre, Armando (Centre de Recerca en Agrigenòmica)
Savoini, Giovanni (Università degli Studi di Milano. Department of Veterinary Medicine and Animal Sciences)
Agazzi, Alessandro (Università degli Studi di Milano. Department of Veterinary Medicine and Animal Sciences)

Date:	2022
Abstract:	Elevated omega-6 (n-6) and omega-3 (n-3) polyunsaturated fatty acids (PUFAs) ratios in swine diets can potentially impose a higher risk of inflammatory and metabolic diseases in swine. A low ratio between the two omega PUFAs has beneficial effects on sows' and piglets' production performance and immunity status. At present, there are few studies on how sow nutrition directly affects the protein and fat deposition in suckling piglets. Two groups of sows were fed diets with high or low n-6/n-3 polyunsaturated ratios of 13:1 (SOY) and 4:1 (LIN), respectively, during gestation and lactation. Longissimus dorsi muscle and adipose tissue from newborn piglets, nourished only with sow's milk, were subjected to fatty acid profiling by gas chromatography-mass spectrometry (GC-MS) and to proteomics assays based on nano-liquid chromatography coupled to high-resolution tandem mass spectrometry (nLC-HRMS). Fatty acid profiles on both muscle and adipose tissues resembled the magnitude of the differences between fatty acid across diets. Proteomic analysis revealed overabundance of 4 muscle and 11 adipose tissue proteins in SOY compared to LIN in both piglet tissues. The detected overabundance of haptoglobin, an acute-phase protein, and the stimulation of protein-coding genes and proteins related to the innate immune response and acute inflammatory response could be associated with the pro-inflammatory role of n-6 PUFAs.
Grants:	European Commission 765423
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Piglets ; Longissimus dorsi ; Adipose tissue ; PUFA ; Proteomics ; Omega-6 ; Omega-3 ; Inflammation ; Fat deposition
Published in:	Molecules, Vol. 27, Issue 9 (May 2022) , art. 2925, ISSN 1420-3049

DOI: 10.3390/molecules27092925
PMID: 35566276

18 p, 729.3 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
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