New insights into flowering date in Prunus : fine mapping of a major QTL in sweet cherry
Branchereau, Camille (Université de Bordeaux. INRAE)
Quero-García, José (Université de Bordeaux. INRAE)
Zaracho, Nathalia (Centre de Recerca en Agrigenòmica)
Lambelin, Laurine (Université de Bordeaux. INRAE)
Fouché, Mathieu (Université de Bordeaux. INRAE)
Wenden, Bénédicte (Université de Bordeaux. INRAE)
Donkpegan, Armel S.L. (Centre INRAE Val de Loire)
Le Dantec, Loïck (Université de Bordeaux. INRAE)
Barreneche, Teresa (Université de Bordeaux. INRAE)
Alletru, David (INRAE. Unité Expérimentale Arboricole (Toulenne))
Parmentier, Julien (INRAE. Unité Expérimentale Arboricole (Toulenne))
Dirlewanger, Elisabeth (Université de Bordeaux. INRAE)

Data:	2022
Resum:	Flowering date is an important trait in Prunus fruit species, especially for their adaptation in a global warming context. Numerous quantitative trait loci (QTLs) have been identified and a major one was previously located on LG4. The objectives of this study were to fine-map this QTL in sweet cherry, to identify robust candidate genes by using the new sweet cherry genome sequence of the cultivar "Regina" and to define markers usable in marker-assisted selection (MAS). We performed QTL analyses on two populations derived from crosses using cultivars "Regina" and "Garnet" as parents. The first one (n = 117) was phenotyped over ten years, while the second one (n = 1386) was evaluated during three years. Kompetitive allele specific PCR (KASP) markers located within the QTL region on LG4 were developed and mapped within this region, consisting in the first fine mapping in sweet cherry. The QTL interval was narrowed from 380 kb to 68 kb and candidate genes were identified by using the genome sequence of "Regina". Their expression was analyzed from bud dormancy period to flowering in cultivars "Regina" and "Garnet". Several genes, such as PavBOI-E3, PavSR45a and PavSAUR71, were differentially expressed in these two cultivars and could be then considered as promising candidate genes. Two KASP markers were validated using a population derived from a cross between cultivars "Regina" and "Lapins" and two collections, including landraces and modern cultivars. Thanks to the high synteny within the Prunus genus, these results give new insights into the control of flowering date in Prunus species and pave the way for the development of molecular breeding strategies.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Horticulture research, Vol. 9 (February 2022) , art. uhac042, ISSN 2052-7276

DOI: 10.1093/hr/uhac042
PMID: 35184200

18 p, 888.4 KB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències > CRAG (Centre de Recerca en Agrigenòmica)
Articles > Articles de recerca
Articles > Articles publicats