The intrinsic chaperone network of Arabidopsis stem cells confers protection against proteotoxic stress
Llamas, Ernesto (University of Cologne)
Torres Montilla, Salvador (Centre de Recerca en Agrigenòmica)
Lee, Hyun Ju (University of Cologne)
Barja, Maria Victoria (Centre de Recerca en Agrigenòmica)
Schlimgen, Elena (University of Cologne)
Dunken, Nick (University of Cologne)
Wagle, Prerana (University of Cologne)
Werr, Wolfgang (University of Cologne)
Zuccaro, Alga (University of Cologne)
Rodríguez Concepción, Manuel (Centre de Recerca en Agrigenòmica)
Vilchez, David (University Hospital Cologne)

Data:	2021
Resum:	The biological purpose of plant stem cells is to maintain themselves while providing new pools of differentiated cells that form organs and rejuvenate or replace damaged tissues. Protein homeostasis or proteostasis is required for cell function and viability. However, the link between proteostasis and plant stem cell identity remains unknown. In contrast to their differentiated counterparts, we find that root stem cells can prevent the accumulation of aggregated proteins even under proteotoxic stress conditions such as heat stress or proteasome inhibition. Notably, root stem cells exhibit enhanced expression of distinct chaperones that maintain proteome integrity. Particularly, intrinsic high levels of the T-complex protein-1 ring complex/chaperonin containing TCP1 (TRiC/CCT) complex determine stem cell maintenance and their remarkable ability to suppress protein aggregation. Overexpression of CCT8, a key activator of TRiC/CCT assembly, is sufficient to ameliorate protein aggregation in differentiated cells and confer resistance to proteotoxic stress in plants. Taken together, our results indicate that enhanced proteostasis mechanisms in stem cells could be an important requirement for plants to persist under extreme environmental conditions and reach extreme long ages. Thus, proteostasis of stem cells can provide insights to design and breed plants tolerant to environmental challenges caused by the climate change.
Ajuts:	European Commission 677427
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Chaperones ; Heat stress ; Plant stem cells ; Protein aggregation ; Protein misfolding ; Proteostasis
Publicat a:	Aging Cell, Vol. 20, Issue 8 (July 2021) , art. e13446, ISSN 1474-9726

DOI: 10.1111/acel.13446
PMID: 34327811

12 p, 2.5 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències > CRAG (Centre de Recerca en Agrigenòmica)
Articles > Articles de recerca
Articles > Articles publicats