Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain
Neila-Ibáñez, Carlos María (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Brogaard, Louise (University of Copenhagen.Department of Veterinary and Animal Sciences)
Pailler-García, Lola (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Martínez Martínez, Jorge (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Segalés Coma, Joaquim (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Segura, Mariela (University of Montreal. Research Group On Infectious Diseases in Production Animals and Swine and Poultry Infectious Diseases Research Centre)
Heegaard, Peter M. H. (Technical University of Denmark. Innate Immunology Group)
Aragon, Virginia (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Data:	2021
Resum:	Streptococcus suis is a zoonotic pathogen of swine involved in arthritis, polyserositis, and meningitis. Colonization of piglets by S. suis is very common and occurs early in life. The clinical outcome of infection is influenced by the virulence of the S. suis strains and the immunity of the animals. Here, the role of innate immunity was studied in cesarean-derived colostrum-deprived piglets inoculated intranasally with either virulent S. suis strain 10 (S10) or non-virulent S. suis strain T15. Colonization of the inoculated piglets was confirmed at the end of the study by PCR and immunohistochemistry. Fever (≥40. 5 °C) was more prevalent in piglets inoculated with S10 compared to T15 at 4 h after inoculation. During the 3 days of monitoring, no other major clinical signs were detected. Accordingly, only small changes in transcription of genes associated with the antibacterial innate immune response were observed at systemic sites, with S10 inducing an earlier response than T15 in blood. Local inflammatory response to the inoculation, evaluated by transcriptional analysis of selected genes in nasal swabs, was more sustained in piglets inoculated with the virulent S10, as demonstrated by transcription of inflammation-related genes, such as IL1B, IL1A, and IRF7. In contrast, most of the gene expression changes in trachea, lungs, and associated lymph nodes were observed in response to the non-virulent T15 strain. Thus, S. suis colonization in the absence of systemic infection induces an innate immune response in piglets that appears to be related to the virulence potential of the colonizing strain.
Ajuts:	European Commission. Horizon 2020 727966
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Streptococcus suis ; Colonization ; Innate immunity ; Pig immunity ; Gene expression ; Bacterial virulence
Publicat a:	Veterinary research, Vol. 52 (december 2021) , ISSN 1297-9716

DOI: 10.1186/s13567-021-01013-w
PMID: 34924012

15 p, 5.1 MB

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