Concordance between Antimicrobial Resistance Phenotype and Genotype of Staphylococcus pseudintermedius from Healthy Dogs
Viñes, Joaquim (Universitat Autònoma de Barcelona. Vetgenomics)
Fábregas, Norma (Universitat Autònoma de Barcelona. Vetgenomics)
Pérez, Daniel (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Cuscó, Anna (Universitat Autònoma de Barcelona. Vetgenomics)
Fonticoba, Rocío (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Francino, Olga (Universitat Autònoma de Barcelona. Vetgenomics)
Ferrer i Caubet, Lluís (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Migura-Garcia, Lourdes (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Date:	2022
Abstract:	Staphylococcus pseudintermedius, a common commensal canine bacterium, is the main cause of skin infections in dogs and is a potential zoonotic pathogen. The emergence of methicillin-resistant S. pseudintermedius (MRSP) has compromised the treatment of infections caused by these bacteria. In this study, we compared the phenotypic results obtained by minimum inhibitory concentration (MICs) for 67 S. pseudintermedius isolates from the skin of nine healthy dogs versus the genotypic data obtained with Nanopore sequencing. A total of 17 antibiotic resistance genes (ARGs) were detected among the isolates. A good correlation between phenotype and genotype was observed for some antimicrobial classes, such as ciprofloxacin (fluoroquinolone), macrolides, or tetracycline. However, for oxacillin (beta-lactam) or aminoglycosides the correlation was low. Two antibiotic resistance genes were located on plasmids integrated in the chromosome, and a third one was in a circular plasmid. To our knowledge, this is the first study assessing the correlation between phenotype and genotype regarding antimicrobial resistance of S. pseudintermedius from healthy dogs using Nanopore sequencing technology.
Grants:	Ministerio de Ciencia e Innovación RTI2018-101991-B-I00 Ministerio de Ciencia e Innovación PTQ2018-009961 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017DI037
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Staphylococcus pseudintermedius ; Antibiotic resistance ; Nanopore sequencing ; Phenotype-genotype concordance ; Healthy dog
Published in:	Antibiotics, Vol. 11 (november 2022) , ISSN 2079-6382

DOI: 10.3390/antibiotics11111625
PMID: 36421269

15 p, 2.7 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Grup de Recerca Malalties infeccioses-inflamatòries en animals de companyia (MIAC)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles