Adenosine a2a receptors are upregulated in peripheral blood mononuclear cells from atrial fibrillation patients
Godoy-Marín, H. (Institut d'Investigació Biomèdica de Bellvitge)
Duroux, R. (Molecular Recognition Section. Laboratory of Bioorganic Chemistry. National Institute of Diabetes and Digestive and Kidney Diseases. National Institutes of Health)
Jacobson, Kenneth A (Molecular Recognition Section. Laboratory of Bioorganic Chemistry. National Institute of Diabetes and Digestive and Kidney Diseases. National Institutes of Health)
Soler, C. (Universitat de Barcelona)
Colino-Lage, Hildegard (Institut d'Investigacions Biomèdiques de Barcelona)
Jiménez-Sábado, Verónica (Institut d'Investigació Biomèdica Sant Pau)
Montiel, José (Institut d'Investigació Biomèdica Sant Pau)
Hove-Madsen, Leif (Institut d'Investigació Biomèdica Sant Pau)
Ciruela, Francisco (Institut d'Investigació Biomèdica de Bellvitge)

Date:	2021
Abstract:	Atrial fibrillation (AF) is the most common form of cardiac arrhythmia seen in clinical practice. While some clinical parameters may predict the transition from paroxysmal to persistent AF, the molecular mechanisms behind the AF perpetuation are poorly understood. Thus, oxidative stress, calcium overload and inflammation, among others, are believed to be involved in AF-induced atrial remodelling. Interestingly, adenosine and its receptors have also been related to AF development and perpetuation. Here, we investigated the expression of adenosine A2A receptor (A2AR) both in right atrium biopsies and peripheral blood mononuclear cells (PBMCs) from non-dilated sinus rhythm (ndSR), dilated sinus rhythm (dSR) and AF patients. In addition, plasma adenosine content and adenosine deaminase (ADA) activity in these subjects were also determined. Our results revealed increased A2AR expression in the right atrium from AF patients, as previously described. Interestingly, increased levels of adenosine content and reduced ADA activity in plasma from AF patients were detected. An increase was observed when A2AR expression was assessed in PBMCs from AF subjects. Importantly, a positive correlation (P=0. 001) between A2AR expression in the right atrium and PBMCs was observed. Overall, these results highlight the importance of the A2AR in AF and suggest that the evaluation of this receptor in PBMCs may be potentially be useful in monitoring disease severity and the efficacy of pharmacological treatments in AF patients.
Grants:	Agencia Estatal de Investigación SAF2017-87349-R Agencia Estatal de Investigación SAF207-88019-C3-1R Agència de Gestió d'Ajuts Universitaris i de Recerca 2017SGR1604 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017SGR1769 Instituto de Salud Carlos III CIBERCV-RD12/0042/0002 Instituto de Salud Carlos III PIE14/00034
Note:	Altres ajuts: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK ZIADK31117)
Note:	Altres ajuts: Fundació La Marató de TV3 (20152030/31)
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Adenosine A2A receptor ; Atrial fibrillation ; Adenosine deaminase ; Peripheral blood mononuclear cells ; Adenosine
Published in:	International journal of molecular sciences, Vol. 22 Núm. 7 (january 2021) , p. 3467, ISSN 1422-0067

DOI: 10.3390/ijms22073467
PMID: 33801676

12 p, 2.9 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles