Taxonomic identification of different species of the genus aeromonas by whole-genome sequencing and use of their species-specific Β-lactamases as phylogenetic markers
Bertran, Xavier (Institut d'Investigació Biomèdica Sant Pau)
Rubio, Marc (Institut d'Investigació Biomèdica Sant Pau)
Gómez Martínez, Laura (Institut d'Investigació Biomèdica Sant Pau)
Llovet, T. (Institut d'Investigació Biomèdica Sant Pau)
Muñoz-Batet, Carmen (Institut d'Investigació Biomèdica Sant Pau)
Navarro Risueño, Ferran (Institut d'Investigació Biomèdica Sant Pau)
Miró, Elisenda (Institut d'Investigació Biomèdica Sant Pau)

Date:	2021
Abstract:	Some Aeromonas species, potentially pathogenic for humans, are known to express up to three different classes of chromosomal Β-lactamases, which may become hyperproduced and cause treatment failure. The aim of this study was to assess the utility of these species-specific Β-lactamase genes as phylogenetic markers using whole-genome sequencing data. Core-genome alignments were generated for 36 Aeromonas genomes from seven different species and scanned for antimicrobial resistance genes. Core-genome alignment confirmed the MALDI-TOF identification of most of the isolates and re-identified an A. hydrophila isolate as A. dhakensis. Three (B, C and D) of the four Ambler classes of Β-lactamase genes were found in A. sobria, A. allosacharophila, A. hydrophila and A. dhakensis (bla, bla and bla). A. veronii only showed class-B- and class-D-like matches (bla and bla), whereas those for A. media, A. rivipollensis and A. caviae were class C and D (bla, bla and bla). The phylogenetic tree derived from concatenated sequences of Β-lactamase genes successfully clustered each species. Some isolates also had resistance to sulfonamides, quinolones and aminoglycosides. Whole-genome sequencing proved to be a useful method to identify Aeromonas at the species level, which led to the unexpected identification of A. dhakensis and A. rivipollensis and revealed the resistome of each isolate.
Note:	Altres ajuts: Departament de Microbobiologia de l'Hospital de la Santa Creu i Sant Pau, i l'Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	MOX ; FOX ; Cephamicinases ; Beta-lactamases ; Aeromonas dhakensis ; Aeromonas rivipollensis ; Core-genome
Published in:	Antibiotics, Vol. 10 Núm. 4 (april 2021) , p. 354, ISSN 2079-6382

DOI: 10.3390/antibiotics10040354
PMID: 33800590

13 p, 916.4 KB

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