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Pàgina inicial > Articles > Articles publicats > Transplantation of human induced pluripotent stem cell-derived retinal pigment epithelium in a swine model of geographic atrophy |
Data: | 2021 |
Resum: | Background: The aim of this study was to test the feasibility and safety of subretinal transplantation of human induced pluripotent stem cell (hiPSC)-derived retinal pigment epithelium (RPE) cells into the healthy margins and within areas of degenerative retina in a swine model of geographic atrophy (GA). Methods: Well-delimited selective outer retinal damage was induced by subretinal injection of NaIO3 into one eye in minipigs (n = 10). Thirty days later, a suspension of hiPSC-derived RPE cells expressing green fluorescent protein was injected into the subretinal space, into the healthy margins, and within areas of degenerative retina. In vivo follow-up was performed by multimodal imaging. Post-mortem retinas were analyzed by immunohistochemistry and histology. Results: In vitro differentiated hiPSC-RPE cells showed a typical epithelial morphology, expressed RPE-related genes, and had phagocytic ability. Engrafted hiPSC-RPE cells were detected in 60% of the eyes, forming mature epithelium in healthy retina extending towards the border of the atrophy. Histological analysis revealed RPE interaction with host photoreceptors in the healthy retina. Engrafted cells in the atrophic zone were found in a patchy distribution but failed to form an epithelial-like layer. Conclusions: These results might support the use of hiPSC-RPE cells to treat atrophic GA by providing a housekeeping function to aid the overwhelmed remnant RPE, which might improve its survival and therefore slow down the progression of GA. |
Ajuts: | Ministerio de Ciencia e Innovación RTI2018-095377-B-100 Instituto de Salud Carlos III PRB2 PT13/0001/0041 Instituto de Salud Carlos III TerCel RD16/0011/0024 Instituto de Salud Carlos III Oftared-RETICS RD16/0008 Instituto de Salud Carlos III AC19/00080 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017-SGR-899 |
Nota: | Altres ajuts: Fondo Europeo de Desarrollo Regional (FEDER); CERCA Programme/Generalitat de Catalunya; Fundació Carmen i Mª José Godó (Fundació CMJ Godó 2017). |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Age-related macular degeneration (AMD) ; Geographic atrophy ; Pig ; Animal model ; Stem cells ; Ipsc ; RPE ; Retina ; Regenerative medicine ; Advanced cell therapy |
Publicat a: | International journal of molecular sciences, Vol. 22 Núm. 19 (October 2021) , p. 10497, ISSN 1422-0067 |
21 p, 6.5 MB |