Iron Induces Resistance Against the Rice Blast Fungus Magnaporthe oryzae Through Potentiation of Immune Responses
Sánchez-Sanuy, Ferran (Centre de Recerca en Agrigenòmica)
Mateluna-Cuadra, Roberto (Centre de Recerca en Agrigenòmica)
Tomita, Keisuke (The University of Tokyo. Biotechnology Research Center)
Okada, Kazunori (The University of Tokyo. Biotechnology Research Center)
Sacchi, Gian Attilio (Università degli Studi di Milano. Dipartimento di Scienze Agrarie e Ambientali)
Campo, Sonia (Centre de Recerca en Agrigenòmica)
San Segundo, Blanca (Centre de Recerca en Agrigenòmica)

Data:	2022
Resum:	Iron is an essential nutrient required for plant growth and development. The availability of iron might also influence disease resistance in plants. However, the molecular mechanisms involved in the plant response to iron availability and immunity have been investigated separately from each other. In this work, we found that exposure of rice plants to high iron enhances resistance to infection by the fungal pathogen Magnaporthe oryzae, the causal agent of blast disease. RNA-Seq analysis revealed that blast resistance in iron-treated rice plants was associated with superinduction of defense-related genes during pathogen infection, including Pathogenesis-Related genes. The expression level of genes involved in the biosynthesis of phytoalexins, both diterpene phytoalexins and the flavonoid phytoalexin sakuranetin, was also higher in iron-treated plants compared with control plants, which correlated well with increased levels of phytoalexins in these plants during M. oryzae infection. Upon pathogen infection, lipid peroxidation was also higher in iron-treated plants compared with non-treated plants. We also show that M. oryzae infection modulates the expression of genes that play a pivotal role in the maintenance of iron homeostasis. Histochemical analysis of M. oryzae-infected leaves revealed colocalization of iron and reactive oxygen species in cells located in the vicinity of fungal penetration sites (e. g. appressoria) in rice plants that have been exposed to iron. Together these findings support that ferroptosis plays a role in the response of iron-treated rice plants to infection by virulent M. oryzae. Understanding interconnected regulations between iron signaling and immune signaling in rice holds great potential for developing novel strategies to improve blast resistance in rice.
Ajuts:	Agencia Estatal de Investigación RTI2018-101275-B-I00 Agencia Estatal de Investigación PID2021-128825OB-I00 Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad CEX2019-000902-S
Nota:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Blast ; Ferroptosis ; Immune response ; Iron ; Magnaporthe oryzae ; Oryza sativa ; Phytoalexins
Publicat a:	Rice, Vol. 15 (December 2022) , art. 68, ISSN 1939-8433

DOI: 10.1186/s12284-022-00609-w
PMID: 36566483

23 p, 3.3 MB

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