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| Pàgina inicial > Articles > Articles publicats > The Role of common cell culture media supplements on citrate-stabilized gold nanoparticle protein corona formation, aggregation state, and the consequent impact on cellular uptake |
(Institut Català de Nanociència i Nanotecnologia) (University of Salzburg. Department of Biosciences) (Universitat Autònoma de Barcelona) (Institut Català de Nanociència i Nanotecnologia) (Hospital Universitari Vall d'Hebron. Institut de Recerca) (Hospital Universitari Vall d'Hebron. Institut de Recerca) (Hospital Universitari Vall d'Hebron. Institut de Recerca) (University of Salzburg. Department of Biosciences) (University of Salzburg. Department of Biosciences) (Institut Català de Nanociència i Nanotecnologia) (Institut Català de Nanociència i Nanotecnologia) (Institut Català de Nanociència i Nanotecnologia)
| Títol variant: | The Role of common cell culture media supplements on the nanoparticle protein corona formation, aggregation state, and the consequent impact on cellular uptake |
| Data: | 2022 |
| Resum: | Sodium citrate-stabilized gold nanoparticles (AuNPs) are destabilized when dispersed in cell culture media (CCMs). This may promote their aggregation and subsequent sedimentation, or under the proper conditions, their interaction with dispersed proteins can lead to the formation of a NP-stabilizing protein corona. CCMs are ionic solutions that contain growth substances which are typically supplemented, in addition to serum, with different substances such as dyes, antioxidants, and antibiotics. In this study, the impact of phenol red, penicillin-streptomycin, l-glutamine, and β-mercaptoethanol on the formation of the NP-protein corona in CCMs was investigated. Similar protein coronas were obtained except in the presence of antibiotics. Under these conditions, the protein corona took more time to be formed, and its density and composition were altered, as indicated by UV-vis spectroscopy, Z potential, dynamic light scattering, and liquid chromatography-mass spectrometry analyses. As a consequence of these modifications, a significantly different AuNP cellular uptake was measured, showing that NP uptake increased as did the NP aggregate formation. AuNP uptake studies performed in the presence of clathrin- and caveolin-mediated endocytosis inhibitors showed that neither clathrin receptors nor lipid rafts were significantly involved in the internalization mechanism. These results suggest that in these conditions, NP aggregation is the main mechanism responsible for their cellular uptake. |
| Ajuts: | European Commission 671881 Agencia Estatal de Investigación RTI2018-099965-B-I00 Agencia Estatal de Investigación BES-2017-083043 Ministerio de Economía y Competitividad SEV-2017-0706 Ministerio de Economía y Competitividad RYC-2012-10991 Instituto de Salud Carlos III PT17/0019 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1431 Agència de Gestió d'Ajuts Universitaris i de Recerca 2016/BP-00350 |
| Nota: | El títol alternatiu correspon al preprint de l'article publicat. |
| Nota: | Altres ajuts: ICN2 is funded by the CERCA Programme/Generalitat de Catalunya. The present work has been performed in the framework of the doctorate in Chemistry and Materials Science at the Autonomous University of Barcelona (UAB). |
| Drets: | Tots els drets reservats.  |
| Llengua: | Anglès |
| Document: | Article ; recerca ; Versió sotmesa a revisió |
| Matèria: | Aggregation state ; Cell culture media ; Cellular uptake ; Clathrins ; Condition ; Gold nanoparticle ; Gold Nanoparticles ; Ionic solutions ; Protein coronas ; Sodium citrate ; Anti-Bacterial Agents ; Cell Culture Techniques ; Citrates ; Citric Acid ; Clathrin ; Gold ; Metal Nanoparticles ; Protein Corona |
| Publicat a: | Bioconjugate chemistry, Vol. 33, issue 8 (Aug. 2022) , p. 1505-1514, ISSN 1520-4812 |
26 p, 1.5 MB |
