Preliminary evaluation of the safety and efficacy of glucose solution infusion through the hepatic artery on irreversible electroporation focusing
Sarreshtehdari, Amirhossein (Universitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut)
Burdío, Fernando (Institut Hospital del Mar d'Investigacions Mèdiques)
López-Alonso, Borja (Universidad de Zaragoza. Departamento de Ingeniería Electrónica y Comunicaciones)
Lucía, Óscar (Universidad de Zaragoza. Departamento de Ingeniería Electrónica y Comunicaciones)
Burdio, José Miguel (Universidad de Zaragoza. Departamento de Ingeniería Electrónica y Comunicaciones)
Villamonte, Maria (Institut Hospital del Mar d'Investigacions Mèdiques)
Andaluz Martínez, Anna (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
García Arnas, Félix (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Berjano, Enrique (Universitat Politècnica de València. Departament d'Enginyeria Electrònica)
Moll, Xavier (Universitat Autònoma de Barcelona. Fundació Hospital Clínic Veterinari)

Date:	2023
Abstract:	Due to electrical features of the tissue, such as impedance, which have a significant impact on irreversible electroporation (IRE) function, the administration of glucose solution 5% (GS5%) through the hepatic artery would focus IRE on scattered liver tumors. By creating a differential impedance between healthy and tumor tissue. This study aimed to determine the effects of the GS5% protocol on healthy liver tissue and its safety. 21 male Athymic nude rats Hsd: RH-Foxn1 mu were used in the study. Animals were split into two groups. In group 1, a continuous infusion through the gastroduodenal artery of GS5% was performed to measure the impedance with a dose of 0. 008 mL/g for 16 min. In group 2, the animals were divided into two subgroups for infusions of GS5%. Group 2. 1, at 0. 008 mL/g for 16 min. Group 2. 2 at 0. 03 mL/g for 4 min. Blood samples were collected after anesthesia has been induced. The second sample, after catheterization of the artery, and the third after the GS5% infusion. All the animals were sacrificed to collect histological samples. The survival rate during the experiment was 100%. A considerable impact on the impedance of the tissue was noticed, on average up to 4. 31 times more than the baseline, and no side effects were observed after GS5% infusion. In conclusion, impedance alteration by Glucose solution infusion may focus IRE on tumor tissue and decrease IRE's effects on healthy tissue.
Grants:	Instituto de Salud Carlos III PI17/0048 Instituto de Salud Carlos III PI21/00440 Ministerio de Economía y Competitividad RTI2018-094357-B-C22 Ministerio de Economía y Competitividad RTI2018-094357-B-C21
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Cancer ; Diseases ; Health care ; Medical research
Published in:	Scientific reports, Vol. 13 (may 2023) , ISSN 2045-2322

DOI: 10.1038/s41598-023-33487-3
PMID: 37130847

9 p, 1.4 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Grup de Recerca Malalties infeccioses-inflamatòries en animals de companyia (MIAC)
Articles > Research articles
Articles > Published articles