Acute psychological stress increases paracellular permeability and modulates immune activity in rectal mucosa of healthy volunteers
Gerdin, Linda (Surgical Clinic of Jönköping County)
González Castro, Ana Maria (Hospital Universitari Vall d'Hebron)
Ericson, Ann-Charlott (Linköping University)
Persborn, Mats (Surgical Clinic of Jönköping County)
Santos, Javier (Hospital Universitari Vall d'Hebron)
Walter, Susanna A. (Linköping University)
Keita, Åsa V. (Linköping University)
Vicario Perez, Maria (Hospital Universitari Vall d'Hebron)
Söderholm, Johan D. (Linköping University)
Universitat Autònoma de Barcelona

Data:	2022
Resum:	Psychological stress and increased permeability are implicated as contributing factors in the initiation and worsening of gastrointestinal diseases. A link between stress and intestinal permeability has been shown in animal models as well as in human small intestine, but stress effects on the human colorectal mucosal barrier has not been reported. To investigate the potential effects of acute psychological stress on colorectal mucosal barrier function and to explore stress-induced molecular events in the rectal mucosa under healthy conditions. Endoscopic biopsies were taken from the rectosigmoid region of healthy volunteers, who had been subjected to dichotomous listening stress and after a control session, respectively. Paracellular and transcellular permeability were assessed in modified Ussing chambers. RNA expression (microarray technology confirmed by quantitative real-time polymerase chain reaction) and biological pathway analysis were used to investigate the local mucosal response to acute stress. Dichotomous listening stress induced a subjective and objective stress response, and significantly increased paracellular but not transcellular permeability. We also identified a stress-induced reduction in RNA expression of genes related to immune cell activation and maturation (CR2, CD20, TCLA1, BANK1, CD22, FDCSP), signaling molecules of homing of immune cells to the gut (chemokines: CCL21, CXCL13, and CCL19, and receptors: CCR7, CXCR5), and innate immunity (DUOX2). Eight of the 10 top down-regulated genes are directly involved in B cell activation, signaling and migration. The systemic stress response correlated positively with paracellular permeability and negatively with DUOX2 expression. Dichotomous listening stress increases paracellular permeability and modulates immune cell activity in the rectal mucosa. Further studies are warranted to identify the primary mechanisms of stress-mediated reduction of mucosal defensive activity and barrier dysfunction, and their potential implications for gastrointestinal disorders.
Ajuts:	Instituto de Salud Carlos III PI19/01643
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Intestinal permeability ; Microarray ; Mucosal immunity ; Psychological stress
Publicat a:	United European Gastroenterology Journal, Vol. 11 (october 2022) , p. 31-41, ISSN 2050-6414

DOI: 10.1002/ueg2.12329
PMID: 36314901

11 p, 1.1 MB

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